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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 455-560-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Clear colourless liquid, purity 96.5%.
- Analytical monitoring:
- no
- Details on sampling:
- A stock solution of 0.5 g/l was prepared by adding 501.3 mg test substance to 1000 ml of Milli-RO water (tap water purified by reverese osmosis; Millipore Corp., Bedford, Mass., USA). Thoroough mixing (15 minutes) was used to accelearte dissolving and to ensure homogeneity. The pH of the clear and colourless stock solution was 8.0. A concentration of 100 mg/l, corresponding to 100 ml of the prepared stock solution in 500 ml final volume, was tested in duplicate.
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 0.5 g/l was prepared, pH 8. The final conc. was 100 mg/l and was tested in duplicate. Inoculum was prepared according to protocol.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Municipal sewarge treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands. The sludge was coarsely sieved, washed and diluted with tap-water. A small amount of the sludge was weighed and dried at ca. 105oC to determine the amount of suspended solids (3.2 g/l of sludge, as used for the test). The pH was 7.5 on the day o fsampling and 7.8 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Test type:
- static
- Water media type:
- other: Tap-water purified by osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges.
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 19.7-19.9 oC
- pH:
- 8
- Dissolved oxygen:
- 7.2-7.7
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- No EC50 could be calculated because pentamethyl-trioxepane is non-toxic EC50 > 100 mg/l
- Results with reference substance (positive control):
- 3,5-dichlorphenol was used as a reference substance. A stock solution of 0.5 g/l was prepared, pH 8. Three conc. were tested 3.2, 10 and 32 mg/l. The EC50 of 3,5-dichlorphenol is between 5-30 mg/l (11 mg/l).
- Validity criteria fulfilled:
- yes
- Remarks:
- The two control respiration rates are within 15% of each other: control 1 and 2, 46 mg/O2/l 4%, control 3 and 4, 43 mg/O2/l 2% and the EC50 of 3,5-dichlorphenol is between 5-30 mg/l (11 mg/l).
- Conclusions:
- Under the conditions of this test pentamethyl-trioxepane was not toxic to activated sludge bacteria at a concentration of 100 mg/l
- Executive summary:
Under the conditions of this testpentamethyl-trioxepane was not toxic to activated sludge bacteria at a concentration of 100 mg/l
Reference
Description of key information
Under the conditions of this testpentamethyl-trioxepane was not toxic to activated sludge bacteria at a concentration of 100 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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