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EC number: 285-378-7 | CAS number: 85085-49-0 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Mentha citrata, Labiatae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity study of aquatic invertebrate for the test chemical :
1)To evaluate short term aquatic toxicity of test material on Daphnia.
2)To evaluate short term toxicity efficiency of test material on aquatic invertebrates. - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material : Mentha citrata, ext.
- IUPAC name : Mentha citrata, ext.
- Molecular formula : C10H18O (Linalool) or C12H20O2 (Linalyl acetate)
- Molecular weight : 154.251- 196.288 g/mol
- Substance type: Organic
- Physical state: Clear liquid (Colorless to pale yellow) - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 1)- Common name: Water flea
- Strain: Straus 1820
-Age at study : ≥24 h old.
2)- Common name: water flea
-Source: CIBA-GEIGY's own testing facility culture
- Age at study initiation: 0-24 h old
- Food type: Scenedesmus subspicatus
supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 14, 48, 72 and 96 h.
- Hardness:
- 1) 240 mg/L (CaCO3)
- Test temperature:
- 1) 21-22°C.
2) 20 +/- 1 °C - pH:
- 1)7.7-8.0
- Dissolved oxygen:
- 1) 94-100%;
- Nominal and measured concentrations:
- 1)10, 18, 32, 58 and 100 mg/L
2)100, 58, 32, 18 and 10 mg/l.
Measured concentrations ranged between 85 and 99% of nominal
at time 0 and between 51 and 72% at time 48 h, the average
of both being between 70 and 81%. - Details on test conditions:
- 1) - Test vessel: beakers
-No. of daphnids: 5/replicates , 4 replicates/treatment
-Static without aeration in the dark; at 20±1°C in beakers, covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L(CaCO3)
2)- Test vessel: Glass vessle
- fill volume: filled with 2.5 L daphnid medium.
- 250 mg linalool was dissolved in and made up with daphnid medium to 2000 ml.
- No. of organisms per vessel: 20 daphnids per concentration
- 4 replicates of 5 daphnids each - Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% CI: 13-17 mg/L)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (53-65 mg/l, 95% CL)
- Details on results:
- EC50 values were calculated according to the maximum likelihood probit model
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical Mentha citrata, ext. is likely to be toxic to aquatic invertebrate in the dose range of 15 - 59 mg/l. Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic invertebrate of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h
>24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.
The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
2.
The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.
Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.
24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.
For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.
Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model
Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
Reference
Description of key information
Short- term toxicity to aquatic invertebrate:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic toxicity of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h
>24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.
The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
2.The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.
Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.
24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.
For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.
Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model
Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 49 mg/L
Additional information
Short- term toxicity to aquatic invertebrate:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic toxicity of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h
>24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.
The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
2.The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.
Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.
24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.
For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.
Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model
Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
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