Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 275-269-2 | CAS number: 71215-81-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991: May 14 to October 8
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Version / remarks:
- (1981)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.9 (Biodegradation: Zahn-Wellens Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- 5-amino-2-chloro-N-(2,4-dimethylphenyl)benzenesulphonamide
- EC Number:
- 275-269-2
- EC Name:
- 5-amino-2-chloro-N-(2,4-dimethylphenyl)benzenesulphonamide
- Cas Number:
- 71215-81-1
- Molecular formula:
- C14H15ClN2O2S
- IUPAC Name:
- 5-amino-2-chloro-N-(2,4-dimethylphenyl)benzene-1-sulfonamide
- Test material form:
- solid
- Details on test material:
- Identity: ACDMA
CAS-No.: 71215-81-1
Solubility in water: slight
Storage Conditlons: At room temperature, in the dark
Safety Precautlons: Routlne hygienic procedures were sufficlent to assure personnel health and safety.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Microorganisms (activated bacterial sludge) from a domestic waste-water sewage plant (ARA, Sissach/Switzerland)
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Based on:
- not specified
- Details on study design:
- Test Medlum
The stock solutions were:
a) 8.5 g KH2PO4, 21.75 g K2HP04, 44.6 g Na2HPO4 x 12H2O, 1.7 g NH4CI dissolved up to 1000 mL bidistilled water.
b) 22.5 g MgSO4 x 7H2O dissolved up to 1000 mL bidistiIIed-water .
c) 27.5 g CaCl2 dissolved up to 1000 mL bidistillled water.
d) 0.25 g FeCl3 x 6H2O dlssolved up to 1000 mL bidlstiIled water.
Three (3) mL each of the stock solutions (a-d) were added up to 1000 mL bidistilled water (final test medium pH adjusted to 7.0).
An amount of sludge fron a domestlc waste-water sewage plant corresponding to 7.5 mg dry material for the positlve control ANILINE and 25.0 mg dry material for the test article were added to 250 mL of the final test medium.
Sanples:
Flask 1: 25O mL bidistilled water and 7.3 mg test article
Flask 2: 25O ml final test medlum, 7.5 mg test article,
Flask 3: 7.4 mg test article,
Flask 4: 7.3 mg test article and 25 mg inoculun.
The pH was adJusted to pH 7.0 before the lnoculum was added.
Flask 13: 25O ml final test redlum, 25 mg ANILINE,
Flask 14: 25 mg ANILINE,
Flask 15: 24 mg ANILINE and 7.5 mg inoculum.
Flask 16: 25O mL flnal test medlum and 25 mg inoculum.
Incubatl on:
Procedure: Inoculated brown glass bottles (500 ml) were lncubated on a BSB 1000 or BSB 501 apparatus (Wissenschaftliche-Technische Werkstätten GmbH, Weilheim/FRG).
Duration: 28 days
Temperature: 25.5 - 27.5°C, Protected from light
Analyses:
Sample preparation:
At the end of the incubation period, samples of about 10 mL from the flasks treated with ACDMA were centrifuged at about 3000g for about 5 minutes and thereafter directly analysed.
T0C-analyses:
T0C-analyses were perforned with the various supernatants, using a Shimadzu 500 TOC-Anal yser.
Cal cul ati ons:
The biodegradability (%BD) was calculated accordlng to the below given formula:
%BD = BOD/(COD or ThOD) x 100%BOD
The valtdtty of the test was evaluated by calculatlon of the biodegradability of the standard compound ANILINE.
Determlnatlon of Theoretlcal Oxygen Demand (ThOD) of ANILINE:
The theoretlcal oxygen demand of ANILINE was 241 mg oxygen per 100 mg ANILINE as calculated from the known molecular weight, assuming the turnover of H into H2O, C into CO2 and N into NH3, accordtng to attachment 2, Directive 84/449 EEC, C.7.
Determlnatlon of Chemical Oxygen Demand (COD) of ACDMA:
The COD was determined according to Directlve 84/49 EEC, C.9.
It was estimated to be 244.94 mg O2/100 mg.
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- other: % biodegradation (BOD/COD)
- Value:
- 15
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Biodegradation (BOD/ThOD) of Aniline: 65.4% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- Inherent biodegradation of ACDMA has been measured at 15% (BOD/COD) within 28 days in test performed accordinf to OECD TG 302C.
- Executive summary:
Inherent biodegradation of ACDMA has been measured at 15% (BOD/COD) within 28 days in test performed accordinf to OECD TG 302C.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.