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EC number: 257-479-6 | CAS number: 51858-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 - 30 Nov 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
Test material
- Reference substance name:
- Dipotassium dihydrogen 4-[5-[3-carboxylato-5-hydroxy-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]penta-2,4-dienylidene]-4,5-dihydro-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazole-3-carboxylate
- EC Number:
- 257-479-6
- EC Name:
- Dipotassium dihydrogen 4-[5-[3-carboxylato-5-hydroxy-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]penta-2,4-dienylidene]-4,5-dihydro-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazole-3-carboxylate
- Cas Number:
- 51858-17-4
- Molecular formula:
- C25H16N4O12S2.2K
- IUPAC Name:
- dipotassium dihydrogen 4-{5-[3-carboxylato-5-oxo-1-(4-sulfonatophenyl)-1,5-dihydro-4H-pyrazol-4-ylidene]penta-1,3-dien-1-yl}-5-hydroxy-1-(4-sulfonatophenyl)-1H-pyrazole-3-carboxylate
- Test material form:
- solid: crystalline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test item concentrations and the control
- Sampling method: Duplicate samples were taken at test start and test end.
- Sample storage conditions before analysis: All samples were stored in a freezer (≤ - 20 °C), protected from light until analysis was performed.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test medium of the highest test concentration of nominal 100 mg test item/L was prepared by dissolving 85.7 mg test item into 857 mL test water by intense stirring for 15 min. Adequate volumes of this test medium were diluted with test water to prepare the test media of the other desired test concentrations.
- Controls: Yes (test medium only)
- Observations: The test medium showed blue coloration in all concentrations.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Clone 5
- Source: in-house laboratory culture
- Age at test start: From 2.25 to 19.25 h old, not first brood progeny.
- Feeding during test: No feeding during the test.
ACCLIMATION
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Green algae (Desmodesmus subspicatus)
- Feeding frequency: At least on all working days.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.5 mmol/L (= 250 mg/L) as CaCO3
- Test temperature:
- 19.7 - 20.0 (Control)
19.6 - 20.2 (Test item concentrations) - pH:
- 7.9
- Dissolved oxygen:
- 8.3 - 8.8 mg/L (Control)
8.5 - 8.9 mg/L (Test item concentration) - Conductivity:
- < 5 μS/cm
- Nominal and measured concentrations:
- Control, 6.3, 12.5, 25, 50 and 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open (covered with glass plate)
- Material, size, headspace, fill volume: 100 mL glass beakers, containing approx 60 mL, 40 mL headspace
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt medium (M4) was used according to the guideline
- Alkalinity: 0.9 mmol/L
- Conductivity: < 5 μS/cm
- Culture medium different from test medium: No
- Intervals of water quality measurement: The water temperature, pH-values and dissolved oxygen concentrations were determined at test start and test end in each treatment group.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Light intensity: 260 to 360 lux
EFFECT PARAMETERS MEASURED: The mobility of the daphnids was determined by visual observation after 24 and 48 h. Those animals that are not able to swim within 15 sec after gentle agitation of the test beaker were considered to be immobile (even if they could still move their antennae).
RANGE-FINDING STUDY: non-GLP pre-experiment
- Results used to determine the conditions for the definitive study: The test item concentrations of the main test were based on the results of a non GLP pre-experiment. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (tested at least twice a year)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 93.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Statistical evaluation was based on two values only:
- Remarks:
- 50 and 100 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no mortality in the controls
- Others: Colouration caused by the test item was observed in all test item concentrations but especially in the highest two test item concentration. - Results with reference substance (positive control):
- The EC50 after 24 h was determined to be 1.58 mg test item/L, indicating that the sensitivity of the daphnids was consistent with the level proposed by the OECD 202 guideline (EC50 (24 h) between 0.6 and 2.1 mg potassium dichromate/L).
- Reported statistics and error estimates:
- The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat® Solutions GmbH.
Any other information on results incl. tables
Analytical Results:
At the start of the test 102% of the nominal test concentrations were found (average of all test concentrations). After 48 h test duration, 102% of the nominal value was determined (average of all test concentrations). During the test the daphnids were exposed to a mean of 102% of nominal. Thus, the effect concentrations were based on the nominal values.
Table 1: Summary of Analytical Results
Sample Description |
% of |
RSD |
|
[mg test item/L] |
nominal 1) |
[%] |
n |
Control |
n.a. |
n.a. |
4 |
6.3 |
109 |
2 |
4 |
12.5 |
104 |
1 |
4 |
25 |
100 |
1 |
4 |
50 |
98 |
1 |
4 |
100 |
99 |
0 |
4 |
1) mean value of all measured samples per treatment group RSD: relative standard deviation per treatment group n: number of analysed samples n.a.: not applicable
Table 2: Biological Results
Nominal Concentration |
No. of Daphnia tested |
No. of immobilized Daphnia after |
% of immobilized Daphnia after |
||
[mg test item/L] |
|
24 h |
48 h |
24 h |
48 h |
Control |
20 |
0 |
0 |
0 |
0 |
6.3 |
20 |
0 |
0 |
0 |
0 |
12.5 |
20 |
0 |
0 |
0 |
0 |
25 |
20 |
0 |
0 |
0 |
0 |
50 |
20 |
0 |
0 |
0 |
0 |
100 |
20 |
0 |
14 |
0 |
70 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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