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EC number: 248-688-3 | CAS number: 27841-06-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 Jul - 05 Jul 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 2010
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- The Department of Health of the Government of the United Kingdom
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Amounts of test item (5, 50 and 500 mg in triplicate) were each separately dispersed in approx. 200 mL of deionised reverse osmosis water and subjected to ultrasonication for approx.. 15 min followed by magnetic stirring for 24 h in order to maximise the dissolved test item concentration. All test vessels were shielded from light during mixing. Synthetic sewage (16 mL), activated sewage sludge (250 mL) and water were added to a final volume of 500 mL to give the required concentrations of 10, 100 and 1000 mg/L (3 replicates). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum: A mixed population of activated sewage sludge micro-organisms was obtained on 04 July 2012 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, UK, which treats predominantly domestic sewage.
- Preparation of inoculum for exposure: The activated sewage sludge sample was maintained on continuous aeration in the laboratory at a temperature of approx. 21 °C overnight prior to use in the test. On the day of collection the activated sewage sludge (5 L) was fed synthetic sewage sludge (250 mL).
- Initial suspended solids concentration: 3.0 g/L prior to use - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- at test start: 6.9 - 7.3
at test end: 7.3 - 7.8 - Dissolved oxygen:
- Control vessels: 5.6 - 6.3 mg/L
Test item vessels: 5.0 - 5.3 mg/L
Reference vessels: 5.5 - 6.1 mg/L - Nominal and measured concentrations:
- Nominal: 0, 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL conical flask
- Measuring vessel: 250 mL darkened glass BOD bottle
- Aeration: air flow of 0.5 - 1 L per minute
- No. of vessels per concentration (replicates): 1 vessel for 10 and 100 mg/L each, 3 vessels for 1000 mg/L
- No. of vessels per control (replicates): 4
- Respiratory substrate: Synthetic sewage with composition as recommended by OECD guideline 209.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test water used was deionised reverse osmosis water containing less than 1 mg/L dissolved organic carbon (DOC).
- Intervals of water quality measurement: The pH of all test solutions was measured at 0 h and after 3 h prior to the measurement of the respiration rate. The oxygen concentrations in all vessels were measured after 30 min contact time.
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Light intensity: normal laboratory lighting
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- The rate of respiration for each flask was measured after 3 hours of incubation.
- Observations were made on the test preparations throughout the test period.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range-finding test: The test reported was planned as range-finding test. As no statistically significant toxic effects were shown at all of the test concentrations employed, it was considered justifiable not to perform a definitive test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - In some instances, the initial and final dissolved oxygen concentrations were outside those recommended in the test guideline. This was considered to have had no adverse effect on the result of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3h) = 7.1 mg/L, 95% CL: 5.6 - 9.0 mg/L - Reported statistics and error estimates:
- The percentage inhibition values were plotted against concentration for the reference item only, a line fitted using the Xlfit software package (IDBS) and the effect concentration values determined from the equation for the fitted line.
95% confidence limits were calculated for the reference item EC50 value using the method of Litchfield and Wilcoxon (1949).
One way analysis of variance incorporating Bartlett’s test for homogeneity of variance and Dunnett’s multiple comparison procedure for comparing several treatments with a control was carried out on the oxygen consumption data after 3 h for the control and all test concentrations to determine any statistically significant differences between the test and control groups. All statistical analyses were performed using the SAS computer software package (SAS, 1999 – 2001). - Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Remarks:
- Summary of available data used for the endpoint assessment of the target substance
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to analogue justification document provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Source: RA-A, CAS 68855-18-5, Croda, 2012, Activated sludge, 3 h, RL2
Referenceopen allclose all
Table 1: Oxygen consumption rates and percentage inhibition values after 3 h contact time
Nominal concentration (mg/L) |
Initial O2 reading (mg O2/L) |
Measurement period (min) |
Final O2 reading (mg O2/L) |
O2 consumption rates (mg O2/L*h) |
% Inhibition |
Control (1) |
3.9 |
3 |
1.5 |
48 |
- |
Control (2) |
3.2 |
2 |
1.6 |
48 |
- |
Control (3) |
3.5 |
2 |
2.0 |
45 |
- |
Control (4) |
3.1 |
2 |
1.5 |
48 |
- |
10 |
3.2 |
2 |
1.5 |
51 |
[8] |
100 |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
1000 (1) |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
1000 (2) |
2.6 |
1 |
1.7 |
54 |
[14] |
1000 (2) |
2.7 |
1 |
1.7 |
60 |
[27] |
n.d., not determined; it was not possible to measure a response over a linear portion of the trace
[Increase in respiration rate as compared to controls]
Table 2: Observations on the test preparations at test start (prior to the addition of activated sewage sludge) and test end
Nominal concentration (mg/L) |
Observations on test preparations |
|
0 hours |
3 hours |
|
Control |
pale yellow/brown dispersion |
dark brown dispersion |
10 |
pale yellow/brown dispersion with slight oily film of test item visible on surface |
dark brown dispersion, no undissolved test item visible |
100 |
pale yellow/brown dispersion with oily film of test item visible on surface |
dark brown dispersion,oily film of test item visible on surface |
1000 |
pale yellow/brown dispersion, large oily globules of test item visible on surface |
dark brown dispersion,oily slick of test item visible on surface |
Reference |
pale yellow/brown dispersion, no undissolved reference item visible |
dark brown dispersion, no undissolved reference item visible |
Description of key information
NOEC (3 h)≥1000 mg/L (nominal; total respiration; activated sludge); no effects up to the limit of water solubility; read-across
Key value for chemical safety assessment
Additional information
There is no study available in which the toxicity of Neopentyl glycol dicaprate (CAS No. 27841-06-1) to microorganisms was assessed. Therefore, read-across to the structurally and chemically closely related source substance Heptanoic acid, ester with 2,2-dimethyl-1,3-propanediol (CAS 68855-18-5) was performed in accordance with Regulation (EC) No 1907/2006 Annex XI, section 1.5 in order to fulfill the data requirements for this substance. The source substance is characterized by similar chemical structure and is therefore considered a suitable representative for the assessment of the toxicity of the target substance to microorganisms. A detailed read-across justification in provided in IUCLID section 13.
The study conducted with the source substance acid, ester with 2,2-dimethyl-1,3-propanediol (CAS 68855-18-5) to microorganisms was performed under static conditions and according to OECD guideline 209, using activated sludgeof a predominantly domestic sewage as test organism. Test solutions of 10, 100 and 1000 mg/L, plus blank control were prepared. No respiration inhibition was observed in the treatments or control during the 3 h exposure period. Hence, the NOEC (3 h) was determined as≥1000 mg/L (nominal; total respiration).
Based on the available result from a structurally related read-across substance (in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5) which is characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that the target substance Neopentyl glycol dicaprate (CAS No. 27841-06-1) is not toxic to microorganisms.
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