Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 239-044-2 | CAS number: 14970-87-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01-02-2017 to 08-10-2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The definitive test was conducted using a range of concentrations deduced from the preliminary range-finding test: activated sewage sludge micro-organisms were exposed to a series of nominal test concentrations of 9.5, 30.5, 97.7, 312.5 and 1000.0 mg/L. In order to obtain both a NOEC and EC50, five treatment concentrations in a geometric series with 2 replicates were prepared. The test item was dispersed directly in ultra-pure water.
The reference item, 3,5-dichlorophenol, was included at concentrations of 3.2, 10 and 32 mg/L in order to confirm again the suitability of the inoculum (test solutions were prepared with a single replicate from the same stock solution as the range-finding test). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Two inoculum were used for this study: the first one (for the preliminary range-finding test) was obtained on 02 January 2017 and the second one (for the definitive test) on 24 July 2017. Both were sampled from the activated sludge of the biological wastewater treatment plant from Mourenx (France, 64) which handles predominantly domestic sewage.
On arrival at the laboratory, the activated sludge was centrifuged (1000g for 10 minutes) and the solid sewage inoculum pellet was re-suspended in 10L deionised water. 500 mL synthetic sewage feed was then added. Sewage sludge was maintained under aerated conditions for a maximum of 5 days and fed daily with synthetic sewage feed.
In order to obtain the required sludge suspended solids concentration of 3 g/L in the stock solution to be used in the experiments, the solid content of the 10-L sludge solution was determined by removing a sub-sample of homogenised sludge and drying in an oven at approximately 105°C. The suspended solids concentration was subsequently adjusted with the source water until the recommended guideline value of 3 g/L ± 10% is achieved. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- The temperature was monitored throughout the test period.
For the range finding test, the recorded temperatures were as follows:!
- Room 1: mean measured temperature: 20.8°C, min.: 20.2°C, max.: 21.1°C.
- Room 2: mean measured temperature: 21.0°C, min.: 20.8°C, max.: 21.3°C.
The ambient temperature during the definitive test did remain constant at 20°C within ± 2°C: mean measured temperature: 20.6°C, min.: 20.5°C, max.: 20.6°C. - pH:
- Measured pH were all at 7.5, no adjustment was necessary.
- Nominal and measured concentrations:
- Nominale concentrations: 9,5 - 30,5 - 97,7 - 312,5 - 1000 mg/L
- Details on test conditions:
- All solutions were incubated at 20 +/- 2°C in darkness for 3 hours in a temperature controlled room. Solutions were mixed by stirring and aerated with clean, oil-free compressed air via narrow bore glass tubes at a rate of 0.5 to 1.0 liter per minute during 3 hours. At the end of the incubation period, a well-mixed sample of each solution (following their order of preparation) were poured into a BOD bottle in which dissolved oxygen concentrations were measured at 1 min intervals for up to a maximum period of 10 min or until the oxygen concentration felt below 1 mg/L.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 772.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 67.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Total respiration was inhibited at each tested concentrations as described below in the table 1:
- Results with reference substance (positive control):
- The 3h-EC50 for the reference item (21.5 mg/L) passed the validity criterion of the guideline set between 2 and 25 mg/L for total respiration
- Reported statistics and error estimates:
- EC10, EC50, LOEC and NOEC values under the conditions of this test were calculated for the test item: see table 2.
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study was designed to determine the inhibition of respiration rates of an activated sludge by the test item following the procedure described in the corresponding OECD guideline 209.
EC10, EC50, LOEC and NOEC values under the conditions of this test were calculated to be as follows for the test item:
95% confidence level
Lower limit Upper limit
NOEC 30.5 mg/L NA NA
LOEC 97.7 mg/L NA NA
EC10 67.4 mg/L 30.1 150.9
EC50 772.1 mg/L 275.7 2016.3
NA: Not Applicable - Executive summary:
This study was designed to determine the effects of the test item on sewage micro-organisms by measuring the respiration rate in 3 hours test according to the OECD Guideline 209 (July 2010).
The inhibition of oxygen uptake by ammonium oxidation (i.e. nitrification) was not determined, so that only total respiration inhibition was assessed in this study.
The definitive test was conducted at the following nominal concentrations: 9.5, 30.5, 97.7, 312.5 and 1000.0 mg/L.
A reference item (3, 5-dichlorophenol) was tested as a reference inhibitory substance at the following nominal concentrations: 3.2, 10.0 and 32.0 mg/L.
The prepared vessels were aerated for 3h, after which dissolved oxygen concentrations were measured at 1 min intervals for up to a maximum period of 10 min. The respiration rate for each vessel was calculated from the measured values of recorded oxygen concentrations versus time andexpressed as mg O2/L/h for the linear part of the graphs (then converted in mg O2/g of activated sludge dry weight of suspended solids/h).
The definitive test met the validity criteria of the guideline detailed as follows:
- The coefficient of variation of oxygen uptake rate in the controls should be no more than 30% at the end of the definitive test: it was determined to be 7.3%
- The 3h-EC50 for the reference item (21.5 mg/L) passed the validity criterion of the guideline set between 2 and 25 mg/L for total respiration
- The blank controls oxygen uptake was not less than 20 mg oxygen/g of activated sludge (dry weight of suspended solids) in an hour: 25.45 mg O2/g of activated sludge (dry weight of suspended solids)/h
EC10, EC50, LOEC and NOEC values under the conditions of this test were calculated to be as follows for the test item:
95% confidence level
Lower limit
Upper limit
NOEC
30.5 mg/L
NA
NA
LOEC
97.7 mg/L
NA
NA
EC10
67.4 mg/L
30.1
150.9
EC50
772.1 mg/L
275.7
2016.3
NA: Not Applicable
Reference
Table 1:
Test item nominal concentration (mg/L) |
Inhibition of total respiration (%) |
9.5 |
7.3 |
30.5 |
10.7 |
97.7 |
16.0 |
312.5 |
29.7 |
1000.0 |
59.0 |
Table 2:
|
95% confidence level |
||
Lower limit |
Upper limit |
||
NOEC |
30.5 mg/L |
NA |
NA |
LOEC |
97.7 mg/L |
NA |
NA |
EC10 |
67.4 mg/L |
30.1 |
150.9 |
EC50 |
772.1 mg/L |
275.7 |
2016.3 |
Description of key information
This study was designed to determine the effects of the test item on sewage micro-organisms by measuring the respiration rate in 3 hours test according to the OECD Guideline 209 (July 2010).
The inhibition of oxygen uptake by ammonium oxidation (i.e. nitrification) was not determined, so that only total respiration inhibition was assessed in this study.
The definitive test was conducted at the following nominal concentrations: 9.5, 30.5, 97.7, 312.5 and 1000.0 mg/L.
A reference item (3, 5-dichlorophenol) was tested as a reference inhibitory substance at the following nominal concentrations: 3.2, 10.0 and 32.0 mg/L.
The prepared vessels were aerated for 3h, after which dissolved oxygen concentrations were measured at 1 min intervals for up to a maximum period of 10 min. The respiration rate for each vessel was calculated from the measured values of recorded oxygen concentrations versus time andexpressed as mg O2/L/h for the linear part of the graphs (then converted in mg O2/g of activated sludge dry weight of suspended solids/h).
The definitive test met the validity criteria of the guideline detailed as follows:
- The coefficient of variation of oxygen uptake rate in the controls should be no more than 30% at the end of the definitive test: it was determined to be 7.3%
- The 3h-EC50 for the reference item (21.5 mg/L) passed the validity criterion of the guideline set between 2 and 25 mg/L for total respiration
- The blank controls oxygen uptake was not less than 20 mg oxygen/g of activated sludge (dry weight of suspended solids) in an hour: 25.45 mg O2/g of activated sludge (dry weight of suspended solids)/h
EC10, EC50, LOEC and NOEC values under the conditions of this test were calculated to be as follows for the test item:
|
95% confidence level |
||
Lower limit |
Upper limit |
||
NOEC |
30.5 mg/L |
NA |
NA |
LOEC |
97.7 mg/L |
NA |
NA |
EC10 |
67.4 mg/L |
30.1 |
150.9 |
EC50 |
772.1 mg/L |
275.7 |
2016.3 |
NA: Not Applicable
Key value for chemical safety assessment
- EC50 for microorganisms:
- 772.1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.