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EC number: 224-638-6 | CAS number: 4433-79-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study report contained sufficient data for evaluation.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Version / remarks:
- according to the draft of November 1978
- Deviations:
- yes
- Remarks:
- Difference to test standard: at the time of the test the use of a reference substance to control the sensitivity of the used Pseudomonas strain was not yet a standard procedure. To the final guideline: temperature
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4'-chloro-2',5'-dimethoxyacetoacetanilide
- EC Number:
- 224-638-6
- EC Name:
- 4'-chloro-2',5'-dimethoxyacetoacetanilide
- Cas Number:
- 4433-79-8
- Molecular formula:
- C12H14ClNO4
- IUPAC Name:
- N-(4-chloro-2,5-dimethoxyphenyl)-3-oxobutanamide
- Details on test material:
- - Name of test material (as cited in study report): Acetessig-2,5-dimethoxy-4-chloranilinide
- Analytical purity: > 99% (CoA, April 1988)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Preparation of stocksolution: not mnentioned in the alabaroty manuscript
- Addition of test substance: 0.5 g/ l in de-ionized water, following by stirring (24 h)
- pH-Adjustment: finally 7.3
- Filtration due to undissolved matter.
- DOC analysis stock solution: 105 mg C/l, according to 198 mg test substance / l (calculated from C-content 53.05% C in the formula)
- final dilution with bacterial suspension: 1+1, corresponds to approx. 100 - 110 mg/l test substance in the test solution
- (Use of) reference substance: no data
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Origin strain: Pseudomonas putida MIGULA, DSM 50026, by Deutsche Sammlung Mikroorganismnen, Mascheroder Wer 1b, D-38124 Braunschweig.
- Laboratory pre culture: according to DIN standard cited
- Acclimitasation to test substance: no
- Initial biomass concentration: Diluted to a turbidity of 5 FAU (formazine attenuation units)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- +- 1 h
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 21-22 ° C followed the DIN method cited. In the final ISO standard method a temperature of 25 - 30° C is required.
- pH:
- pH stock solution prior to dilution: 7.3
- Dissolved oxygen:
- Din standard medium
- Salinity:
- Din standard medium
- Nominal and measured concentrations:
- Measured concentration DOC stock solution: 105 mg C /l, corresponds to 198 mg test substance / l, after dilution with bacteria suspension approx. 100 - 110 mg/ l
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 110 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- other: DOC
- Basis for effect:
- growth inhibition
- Remarks:
- measured by absorption
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 110 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- other: DOC
- Basis for effect:
- growth inhibition
- Remarks:
- measured by absorption
- Details on results:
- Measures taken 16 h + - 1 h
- Results with reference substance (positive control):
- no data of use of reference substance in parallel to test substance
Any other information on results incl. tables
Absorption at 436 nm
|
Dilution Factor (F) |
Mean * F |
Inhibition |
||
Blank 1 |
0.247 |
0.221 |
10 |
2.434 |
|
Blank 2 |
0.247 |
0.231 |
|||
Blank 3 |
0.271 |
||||
test substance |
0.252 |
0.260 |
10 |
2.560 |
0 % |
where Inhibition = absorbance (blank) – absorbance (substance) / absorbance (blank) – absorbance (inoculum)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- At the tested concentration of approx. 100 - 110 mg test substance / l bacterial suspension no significant inhibition could be observed.
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