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EC number: 212-506-0 | CAS number: 822-89-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-03-02 to 1995-03-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydroxyl-2-pyridone
- EC Number:
- 212-506-0
- EC Name:
- Hydroxyl-2-pyridone
- Cas Number:
- 822-89-9
- Molecular formula:
- C5H5NO2
- IUPAC Name:
- 1-hydroxy-1,2-dihydropyridin-2-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (as cited in study reports): Oxy PYRION
- Physical state: solid (powder)
- Appearance: white speckled gold powder
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor, batch 93100020
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Stability under test conditions: stable
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Pre-test (Closed bottle test (OECD 301D):
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Thorndon sewage treatment works, secondary effluent, trickling filter plant that treats predominantly domestic waste, collected at day of use.
- Storage conditions: maintained under aerobic conditions until use and vacuum filtered
- Storage length: less than a day
- Preparation of inoculum for exposure:
- Concentration of sludge: 30 mg/l total suspended solids used in test
- Water filtered: yes
- Type and size of filter used, if any: Watman GFC
Pre-test (CO2 evolution test, OECD 301B):
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Oakley STW, activated sludge, predominantly domestic waste, collected the day before the test.
- Storage conditions: aerated in the lab for four hours
- Storage length: less than a day
- Preparation of inoculum for exposure: the activated sludge was homogenized in a mechanical blender and aliquots were filtered through dried and preweighted Whatman GFC filter papers which were dried at 110 °C for at least one hour, allowed to cool in a desiccator and reweighed.
- Concentration of sludge: 30 mg/l total suspended solids used in test
- Water filtered: yes
- Type and size of filter used, if any: Watman GFC
Main test (OECD 301B):
Methods for preparing the inoculum and conducting the test are similar to those described for the OECD 301B pre-test. - Duration of test (contact time):
- ca. 29 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 12 mg/L
- Based on:
- TOC
- Initial conc.:
- 22.2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Standard Mineral salts medium (MSMS)
- Test temperature: 21.3 to 23.3°C
- pH: 7.5 to 7.6 at the start of the test and 7.4 to 7.6 at the end
- pH adjusted: yes, with 5N NaOH
- Aeration of dilution water: yes
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: air flushed into vessel from inlet under the stopper; outlet contected to 3 Dreschel bottles in series containing 0.025N BaOH (100 mL); air flow 33-43 mL/min
- Test performed in closed vessels due to significant volatility of test substance: no
- Details of trap for CO2 and volatile organics if used: Dreschel bottles containing 0.025N BaOH
SAMPLING
- Sampling frequency: Day 4, 8, 12, and 29
- Sampling method: not specified
- Sample storage before analysis: not specified
CONTROL AND BLANK SYSTEM
- Inoculum blank: inoculated MSMS
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- the study was preceed by a solubility test and a 5-day bacterial inhibition assay conducted according to the conditions of the closed bottle test (OECD 301D): oxypryrion showed inhibition to the weak bacterial inoculum at a nominal concentration of 22.2 mg/L (12 mg C/L)
A second inhibition assay was conducted under the conditions of the CO2 evolution test (OECD 301B): oxypyrion did not significantly inhibit the activity of the bacterial inoculum at nominal concentrations of 6 and 12 mg C/L.
The main OECD 301B test was conducted at a nominal oxypyrion concentration of 12 mg C/L - Test performance:
- The rate of degradation of sodium benzoate (60% of its TCO2 after four days and 94% after 29 days) and cumulative CO2 production in the control (84.1 and 79.1 mgCO2) fulfill the validity criteria for this test.
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 20
- Sampling time:
- 8 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 61
- Sampling time:
- 12 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 94
- Sampling time:
- 29 d
- Details on results:
- - concentrations of oxypyrion measured by HPLC analysis of duplicate samples from each test mixture were 22.8 and 22.9 mg/L (103% of nominal value).
BOD5 / COD results
- Results with reference substance:
- The positive control item was biodegraded by at least 60% within 14 days (60% after 4 days), confirming suitability of the activated sludge.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- A 28-d ready biodegradability test (OECD 301B, modified Sturm test) using unadapted activated sludge from a predominantly domestic waste water treatment plant indicated that Oxypyrion is readily biodegradable under the conditions of the test (initial concentration 12 mgC/L). The test substance showed 20% after 8 days of incubation, 61% after 12 days and 94% degradation after 29 days of incubation. The results of the test can be considered reliable without restriction.
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