Registration Dossier
Registration Dossier
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EC number: 209-578-0 | CAS number: 586-62-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
An acute aquatic toxicity study was performed on Daphnia magna in accordance with OECD Guideline No 202.
The 48h-EC50 of terpinolene monoconstituent for freshwater invertebrates is 0.634 mg/L.
Based on the results of this study, terpinolene monoconstituent is classified:
- dangerous for the environment according to Directive 67/548/EEC "R50/53: very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment"
- and hazardous to the aquatic environment under Regulation (EC) No 1272/2008 (CLP) - Category Acute 1 and Category Chronic 1.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.634 mg/L
Additional information
Key study conducted according to OECD 202 Guideline and EU Method C.2 under GLP without deviation.
Daphnids were exposed to terpinolene monoconstituent at nominal concentrations of 0.2, 0.3, 0.4, 0.5, 0.7 and 1.0 mg/L. Immobilisation of daphnids was recorded in a semi-static 48-hour test by visual observation after 24 and 48 hours. Concentrations were measured at the start of the study, at 24 h (new and old solutions) and at the end of the test. The concentrations of the test item were not satisfactorily maintained within ± 20% of the initial concentrations throughout the test, thus the EC50 were based on the geometric mean of the measured concentrations. The 48h-EC50 determined in this study can be used as key value for chemical safety assessment.
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