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EC number: 203-737-8 | CAS number: 110-12-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted under GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Approximately 4 L of activated sludge was collected from the Wareham, Massachusetts Wastewater Treatment Plant which treats primary domestic sewage. Upon arrival at Springborn. the activated sludge was centrifuged at 1000 rpm for 10 minutes. The supernatant (or secondary effluent) was kept and the solids discarded. The resultant secondary effluent was filtered through coarse filter paper. Following filtration, the first 200 mL of secondary effluent was discarded. The next 500 mL of secondary effluent was retained and aerated. The inoculum was used on the same day as it was prepared.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 7 mg/L
- Based on:
- ThOD/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Oxygen-rich water was prepared by bubbling compressed air through water for 20 minutes. Nutrient solutions were prepared using the oxygen-rich water. The nutrient solutions were then aerated for approximately 20 minutes and stored in a refrigerator until used. The water was aerated for an additional 20 minutes and was allowed to stand overnight at 20 ± 1 °C prior to test initiation. Fifty-four industrial 300-mL BOD bottles were prepared for the reference and test substances and the inoculum control blanks; eighteen bottles per treatment, two each for day 0, three per sampling interval (i.e., days 7, 14, 21 and 28) and an additional four bottles for each treatment. The filled BOD bottles were maintained in a dark environmental chamber, set to maintain a temperature of 22 ± 2 °C. A 2.5 mg/L [test substance] solution was prepared by adding 15 mg (18.75 µL) of the test substance directly into 6 L of mineral media. A 10 mg/mL sodium benzoate stock solution was prepared by adding 1.0112 g (1.001 g as a.i.) of sodium benzoate to a flask and diluting to a volume of 100 mL with sterile reagent grade water. The theoretical oxygen demand (ThOD) for the test substance was calculated to be 2.80 mg O2/mg. A nominal test substance concentration of 2.5 mg/L was selected as the test concentration. The test solutions were prepared by first adding approximately 4 L of the aerated water by siphon to each of three 9-L bottles, one for the test substance, one for the sodium benzoate and one for the inoculum control blank. Each 9-L bottle then received 6 mL of each of the four nutrient solutions. The test substance bottle next received 960 µL of the secondary effluent and 18.75 µL of the 2.5 mg/L test substance solution, the sodium benzoate bottle received 960 µL of the secondary effluent and 3.0 mL of the 10 mg/mL sodium benzoate stock solution and the inoculum blank bottle received 960 µL of the secondary effluent. Each of the three bottles was then brought to a final volume of 6 L with the aerated water. Immediately after preparation, each solution was siphoned (bubble-free) from the 9-L bottles into the respective series of 18 BOD bottles (for a total of 54 bottles for the three solution types). The bottles were slightly overfilled to eliminate all air bubbles. Three bottles from each set were labeled for each interval (i.e., days 7. 14, 21 and 28) and two bottles from each set were labeled for day 0. Once filled, the bottles were closed, wrapped in foil and labeled.
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 55
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 61
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 67
- Sampling time:
- 28 d
- Results with reference substance:
- Degradation in the sodium benzoate test solutions was greater than 60 % by day 10 and 72 % on day 28 which indicated that the inoculum contained a viable microbial population and that the test procedures used during the study were adequate.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Executive summary:
The determination of the ready biodegradability of methyl isoamyl ketone following OECD Guideline 301 D (Closed Bottle Test) was conducted. 61 % degradation of the test substance occurred by day 14 and 67 % degradation was observed by termination of the 28-day study. The test substance can be classified as readily biodegradable under the conditions of this test.
Reference
Description of key information
The determination of the ready biodegradability of methyl isoamyl ketone following OECD Guideline 301 D (Closed Bottle Test) was conducted. 61 % degradation of the test substance occurred by day 14 and 67 % degradation was observed by termination of the 28-day study. The test substance can be classified as readily biodegradable under the conditions of this test.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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