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EC number: 200-554-5 | CAS number: 63-05-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Water solubility (under test conditions): < 100 mg/L
- Analytical monitoring:
- yes
- Details on sampling:
- Sampies for the concentration analysis were taken at time points 0 (measurement vessel) and
48 hours (all test vessels) after start of exposure. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Schering AG, Institute for Experimental Toxicology
- Age at study initiation (mean and range, SD): 0 - 24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
- Test temperature:
- 19.5 - 20.7°C
- pH:
- 7.9 - 8.5
- Dissolved oxygen:
- 5.3 - 8.9 mg/L
- Nominal and measured concentrations:
- nominal: 0.9, 2.0, 4.5, 10, 22, 48 mg/L
mean measured: 1.0, 2.3, 4.4, 8.7, 17.2, and 37.0 mg/L - Details on test conditions:
- 20 Daphnia were used for each test solution and 20 tor the dilution water control, randomly distributed into groups of five. LabelIed 100 ml test vessels were filled with 45 ml of the test solution or tap water (contral group) and 5 mL of the dilution water with the introduction of the Daphnia. The test was performed with 4 replicates of each test solution and the dilution water contral with 5 Daphnia in each vessel. One additional test vessel for the test concentration and the control solution, respectively, was used without Daphnia for the measurements of pH, temperature and oxygen content.
The Daphnia were exposed to the test solutions and the dilution water for a period of 48 hours under static conditions. The light/dark rhythm was adjusted to 16 hours/8 hours. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 17.6-26.8
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Cumulative immobilization:
Concentration of ZK 5155 No. of Daphnia No. of immobilized Daphnia
(mg/L) 24 hours 48 hours
0 20 0 1
1.0 20 0 0
2.3 20 0 1
4.4 20 0 2
8.7 20 0 3
17.2 20 3 5
37.0 20 2 19 - Reported statistics and error estimates:
- The EC 50 was calculated with a computer programme based on probit analysis.
- Validity criteria fulfilled:
- yes
- Remarks:
- immobilisation and other abnormalities in the controls did not exceed 10 % by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period
- Conclusions:
- After 48 h an EC-50 of 21.4 mg/L was measured for Androst-4-ene-3,17-dione towards Daphnia. The NOEC for 48 h was 1.0 mg/L, the LOEC was 2.3 mg/L. The results are relating to measured concentrations.
- Executive summary:
Acute toxicity to Daphnia magna was determined in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). 20 Daphnia each were exposed to the different test concentrations of androstedione (1.0, 2.3, 4.4, 8.7, 17.2, and 37.0 mg/L; mean measured) over 48 hours. The pH, temperature and O2was 7.9-8.5, 19.9-20.7 C and 5.3-8.9 mg/L, respectively. The concentration of Androst-4-ene-3,17-dione was measured by HPLC/UV at 0 and 48 hours. The EC50 was calculated based on probit analysis.
The 48 h-EC50 was 21.4 mg/L (measured concentration). At 24 hours, only 10-15% immobilized daphnids were observed at the two highest concentrations. The NOEC for 48 h was 1.0 mg/L, the LOEC was 2.3 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.
Reference
One immobilized Daphnia magna was observed in the control group after 48 hours. An immobilization of one individual (= 5%) is within the limit accepted by the OECD-Guideline for the control group. At 24 hours, only 10 -15% immobilized daphnids were observed at the two highest concentrations.
Validity criteria for the measurement of the short-term toxicity to aquatic invertebrates:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Young daphnids, aged less than 24 hours at the start of the test, are exposed to the test substance at a range of concentrations for a period of 48 h. | Neonates were used in this test. | Yes |
In the control, including the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | In the control, one immobilized daphnid was found. | Yes |
At least five test concentrations should be used. They should be arranged in a geometric series with a separation factor preferably not exceeding 2.2. The highest concentration tested should preferably result in 100 % immobilization, and the lowest concentration tested should preferably give no observable effect. | Six test concentrations were used. | Yes |
Description of key information
After 48 h an EC-50 of 21.4 mg/L was measured for Androst-4-ene-3,17-dione towards Daphnia. The NOEC for 48 h was 1.0 mg/L, the LOEC was 2.3 mg/L. The results are relating to measured concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 21.4 mg/L
Additional information
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