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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP non-guideline, experimental study, published in peer reviewed literature, predates implementation of GLP and/or development of study guideline but otherwise acceptable for assessment.
Qualifier:
no guideline followed
Principles of method if other than guideline:
Continuous flow bioassay method
GLP compliance:
no
Remarks:
Predates GLP
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
No data reported
Analytical monitoring:
yes
Details on sampling:
No data
Details on test solutions:
Stock solutions of individual compounds were made by mixing a known quantity of compound in each 208l drum.
Test organisms (species):
Carassius auratus
Details on test organisms:
All fish 13-20cm length, weight 20-80g obtained from Grassy Fork Fisheries in Martinsville, Indiana, USA. Standard Method (1965), was used as a guideline for the preparation of fish before experimentation.
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No data reported
Hardness:
80ppm as CaCO3
Test temperature:
7.0 ± 0.3
pH:
17-19⁰C
Dissolved oxygen:
≥7ppm
Salinity:
No data reported
Nominal and measured concentrations:
No data reported
Details on test conditions:
No data reported
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
20.57 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL 17-72 - 25.26 mg/l
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
16.17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 955CL 13.85-18.7 mg/l1
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
13.65 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%CL 11.21-16.15 mg/l
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
12.52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%CL 9.89-15.05 mg/l
Details on results:
LC50(24hrs) 20.57 (17.72-25.26), LC50(48hrs) 16.17 (13.85-18.71), LC50(72hrs) 13.65 (11.21-16.15), LC50(96hrs) 12.52 (9.89-15.05) mg/l.
Results with reference substance (positive control):
All control fish survived the time duration of each bioassay.
Reported statistics and error estimates:
95% confidence limit
Validity criteria fulfilled:
not applicable
Conclusions:
The reported 96hr LC50 for Carassius auratus (Goldfish) was experimentally determined to be 12.52 mg/L. The result does not indicate that mesitylene (1 ,3 ,5-trimethylbenzene) would meet the criteria for toxic in the PBT assessment. This endpoint will not effect the classification of the substance since a lower LC50 is found for Daphnia magna which will determine the classification.
Executive summary:

This study has a reliability rating of 2 because the source predates implementation of GLP and development of study guidelines, however the procedure and means of analysis suggest that the methodology was appropriate to evaluate the short term toxicity to fish. There is otherwise no information in the article to suggest that the data are invalid. The reported 96hr LC50 for Carassius auratus (Goldfish) was experimentally determined to be 12.52 mg/L. The result does not indicate that mesitylene (1, 3, 5-trimethylbenzene) would meet the criteria for toxic in the PBT assessment. This endpoint will not effect the classification of the substance since a lower LC50 is found for Daphnia magna which will determine the the classification.

Description of key information

The experimentally determined 96 hour LC50 for Carassius auratus (Goldfish) was reported to be 12.52 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
12.52 mg/L

Additional information

The Brinniman et al (1976) study predates implementation of GLP and development of study guidelines, however the procedure and means of analysis suggest that the methodology was appropriate to evaluate the short term toxicity to fish. There is otherwise no information in the article to suggest that the data are invalid. The experimentally determined 96 hour LC50 for goldfish was reported to be 12.52 mg/L.

A supporting QSAR calculation using the ECOSAR model gives an estimated 96 hour LC50 for fish of 5.2 mg/L. The CITI (1981) study was given a reliability of 4 because the primary source was unavailable for review. When exposed to 1,3,5-trimethylbenzene, the 48 hour LC50 for Oeyzias latipes (Rice Fish)) was experimentally determined to be 8.6 mg/L.