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EC number: 434-430-9 | CAS number: -
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviation from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- column elution method
- Water solubility:
- < 0.012 mg/L
- Temp.:
- 20 °C
- Remarks on result:
- other: Peak 1. pH was 4.2 and 6.0 for the two different flow rates.
- Water solubility:
- < 0.03 mg/L
- Temp.:
- 20 °C
- Remarks on result:
- other: Peak 2. pH was 4.2 and 6.0 for the two different flow rates.
- Water solubility:
- < 0.1 mg/L
- Temp.:
- 20 °C
- Remarks on result:
- other: Peak 3. pH was 4.2 and 6.0 for the two different flow rates.
- Conclusions:
- Interpretation of results (migrated information): insoluble (< 0.1 mg/L)
Since EA-3098 is a mixture of components, quantitative analysis was based on the three largest peaks in the chromatogramn of EA-3098.
The water solubility of EA-3098 at 20.4 ± 0.5°C is < 1. 2x 10-5 g/l based on peak 1, < 3.0 x 10-5 g/l based on peak 2 and < 1.0 x 10-4 g/l based on peak 3, using the column elution method.
The pH was measured to be 4.2 and 6.0. - Executive summary:
The column elution method was used to determine the water solubility of EA-3098 and was based on the following guidelines:
OECD Guideline no. 105: "Water Solubility" and EEC Directive 62/69/EEC Method A.6 "Water solubility".
Since EA-3098 is a mixture of components, quantitative analysis was based on the three largest peaks in the chromatogramn of EA-3098.
The water solubility of EA-3098 at 20.4 ± 0.5°C is < 1.2 x 10-5 g/l based on peak 1, < 3.0 x 10-5 g/l based on peak 2 and < 1.0 x 10-4 g/l based on peak 3, using the column elution method.
The pH was measured to be 4.2 and 6.0.
.
Reference
Preliminary test:
The concentration of EA-3098 in the aqueous phases after two and three successive centrigugation steps was for peak 1 <1.0 x 10 -5 g/l, peak 2 <4.0 x 10-5 g/l and for peak 3 <1.o x 10-4 g/l, respectively. From this it could be concluded that the water solubility of EA-3098 is <10 mg/l. Therefore, the column elution method was chosen for the determination of the water solubility of EA-3098. The pH of the aqueous phase after 2 centrifugation steps was 9.5.
Main study (column elution method):
Analysis confirmed the presence of test substance on the loaded carrier material before filling the column.
The eluates did not contain undissolved particles. In the eluates from the blank column a small peak was detected for peak 1. The concentration was calculated using the RF-factor and was <Limit of Detection (LOD).
The results are summarised in Tables 1, 2 and 3.
Table 1: Results of column elution method for peak 1
Actual flow rate (ml/h) |
Sample no. |
Concentration analysed (mg/l) |
pH |
24 |
1-10 |
<LOD* |
4.2 |
12 |
1-10 |
<LOD* |
6.0 |
* LOD = 0.012 mg/l (all samples contained a small test substance peak, which was smaller than the limit of detection).
Table 2: Results of column elution method for peak 2
Actual flow rate (ml/h) |
Sample no. |
Concentration analysed (mg/l) |
pH |
24 |
1-10 |
<LOD* |
4.2 |
12 |
1-10 |
<LOD* |
6.0 |
*LOD = 0.03 mg/l
Table 3: Results of column elution method for peak 3
Actual flow rate (ml/h) |
Sample no. |
Concentration analysed (mg/l) |
pH |
24 |
1-10 |
<LOD* |
4.2 |
12 |
1-10 |
<LOD* |
6.0 |
*LOD = 0.1 mg/l
Description of key information
Since EA-3098 is a mixture of components, quantitative analysis was based on the three largest peaks in the chromatogramn of EA-3098.
The water solubility of EA-3098 at 20.4 ± 0.5°C is < 1.2 x 10-5 g/l based on peak 1, < 3.0 x 10-5 g/l based on peak 2 and < 1.0 x 10-4 g/l based on peak 3, using the column elution method.
Key value for chemical safety assessment
- Water solubility:
- 0.1 mg/L
- at the temperature of:
- 20.4 °C
Additional information
The column elution method was used to determine the water solubility of EA-3098 and was based on the following guidelines:
OECD Guideline no. 105: "Water Solubility" and EEC Directive 62/69/EEC Method A.6 "Water solubility".
Since EA-3098 is a mixture of components, quantitative analysis was based on the three largest peaks in the chromatogramn of EA-3098.
The water solubility of EA-3098 at 20.4 ± 0.5°C is < 1.2 x 10-5g/l based on peak 1, < 3.0 x 10-5g/l based on peak 2 and < 1.0 x 10-4g/l based on peak 3, using the column elution method.
The pH was measured to be 4.2 and 6.0.
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