Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 436-710-6 | CAS number: 756-13-8
A total of eight aquatic toxicity studies were performed with CAS# 756-13-8. All acknowledged the hydrolytic instability of CAS# 756-13-8 under the test conditions and expressed results in terms of the concentration of its water-soluble hydrolysis product pentafluoropropionic acid (CAS# 422-64-0; perfluoropropionic acid; PFPA). Therefore all equated the effects due to CAS# 756-13-8 with the equivalent mass of PFPA. Seven of these studies were performed by dissolving CAS# 756-13-8 in water and testing the toxicity of the resulting solution, while one examined the toxicity of PFPA directly. The results are summarized in the Table (below).
All studies were performed according to accepted international guidelines for testing of chemical toxicity to algae, fish, and daphnia, and were in compliance with OECD GLP standards. Studies with Pseudokirchneriella subcapitata, Anabaena flos-aquae, and Navicula pelliculosa were performed in accordance with OECD Guideline 201 (Alga, Growth Inhibition Test). Testing in Lemna gibba was performed following EPA OPPTS 850.4400 (Aquatic Plant Toxicity Test using Lemna spp. Tiers I & II). Toxicity in the Water flea (Daphnia magna) was measured using OECD Guideline 202 (Daphnia sp. Acute Immobilization Test). The 96-hr LC50 for Pimephales promelas was determined following OECD Guideline 203 (Fish, Acute Toxicity Test), while toxicity in Oryzias latipes was determined under the Japanese guideline JIS K 0102-1998-71, “Testing methods for industrial waste water, Acute toxicity test with fish,” as a preliminary study to the determination of the bioconcentration of CAS# 756-13-8 / PFPA in fish.
These tests yielded time-appropriate EC50/LC50 values ranging from ~6.8 mg/L to >1080 mg/L for aquatic organisms. Four were assigned Klimish rankings of 1 (reliable without restriction), three were assigned rankings of 2 (reliable with restrictions), and one was assigned a ranking of 3 (not reliable).
Of note, two studies were performed measuring average specific growth rate in the green algae Pseudokirchneriella subcapitata (formerly Selanastrum capricornutum). One study examined the toxicity of PFPA generated from CAS# 756-13-8 in a separate vessel and added to the test solutions, while the other added CAS# 756-13-8 directly to the test solutions. The 96-h EC50 values from the two studies were 10.6 mg/L and > 6.78 mg/L respectively. Using data from these same two studies, 96-h NOEC values were determined to be 3.71 and 3.47 mg/L, again, respectively. The geometric mean of the EC50 values was used to calculate the PNEC for CAS# 756-13-8 which was used in conjunction with the environmental distribution to determine the risk characterization ratio for CAS# 756-13-8 / PFPA.
Aquatic Toxicity Studies on CAS# 756-13-8 and its hydrolysis product PFPA
Nominal test substance
Class of organism
End points (expressed as concentration of PFPA)
Analysis of test substance
PFPA from CAS# 756-13-8
algae & freshwater plants (green algae)
Average specific growth rate:EC50(96h) = 10.6 mg/LNOEC(96h) = 3.71 mg/LCell count:EC50(96h) = 5.37 mg/LNOEC(96h) = 1.57 mg/L
Average specific growth rate:EC50(72&96h) > 6.78 mg/LNOEC(96h) = 3.47 mg/L
algae & freshwater plants (blue-green algae)
Average specific growth rate:EC50(72&96) > 86.6 mg/LNOEC (96h) = 44.0 mg/L
algae & freshwater plants (diatom)
Average specific growth rate:EC50(72&96h) > 14.2 mg/LNOEC(72&96h) = 14.2 mg/L
algae & freshwater plants (green plant)
EC50 (7d) > 17.7 mg/LNOEC (7d) Not calculated
EC50(48h) > 1080 mg/LNOEC - not determined
LC50(96h) > 1070 mg/LNOEC(96h) = 1070 mg/L
LC50(96h) = 408 mg/LNOEC(96h) = 333 mg/L(NOEC estimated by author of RSS; not estimated in report from testing organization
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again