Nickel, 5-[2-(2-hydroxy-3,7-disulfo-1-naphthalenyl)diazenyl]-1H-1,2,4-triazole-3-carboxylate sodium complexes

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test phase 2002-10-28 to 2002-11-01. Study report 2002-12-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

443-510-2

EC Name:

-

Cas Number:

738587-10-5

Molecular formula:

C26H14N10Ni2O18S4 as free acid

IUPAC Name:

dinickel(2+) sodium 3-carboxy-5-[(1E)-2-(7-oxido-2,6-disulfonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide 3-carboxy-5-[(1E)-2-(7-oxido-2-sulfo-6-sulfonatonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The study was run with nominal concentrations of 1500 mg/L of test material together with a dilution water control.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Mirror carp
- Source: Reputable supplier
- Length at end of study: 36-40mm, mean 38mm
- Weight at end of study: 1.21-2.57g, mean 1.87g
- Feeding during test: none

ACCLIMATION
- Acclimation period: 7d
- Acclimation conditions: 22 +/- 2 degrees C
- Type of food: commercial fish food (food was witheld at least 24h prior to commencement of test)
- Health during acclimation:No mortalities observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Hardness of dilution water 41.7 mg/L CaCO3

Test temperature:

21.8-22.2 degrees C

pH:

7.7-8.3

Dissolved oxygen:

89-94%

Salinity:

Salinity of dilution water 1.0

Nominal and measured concentrations:

The overall mean measured concentration was 107% of the nominal value. The percentage loss in the measured concentrations over the test period was <1%. On the basis of the analytical data the nominal concentrations were used for the calculation and reporting of results.

Details on test conditions:

TEST SYSTEM
- Test vessel: Borosilicate glass
- Fill volume: Vessels had a max. capacity of 40L and a working volume of 25L
- Aeration: Test solutions were gently aerated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- Biomass loading rate: 0.75g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water which has been passed through activated carbon, coarsely filtered, dechlorinated, salts added (to maintain hardness), UV sterilised and re-filtered (5um).
- Chlorine (as Cl2): Free residual <2 ug/L Combined residual <2 ug/L
- Alkalinity: 20.4 mg/L (as CaCO3)
- Conductivity: 243 uS/cm (at 25 degrees C)
- Intervals of water quality measurement: Daily measurements of the test solutions for pH, temperaure and dissolved oxygen. The temperature of the dilution water control was measured hourly.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours fluorescent light and 8 hours dark with a 20 min. dawn/dusk transition period.

EFFECT PARAMETERS MEASURED: Observation for mortalities were made at 3, 24, 48, 72 and 96 h.

TEST CONCENTRATIONS
- Test concentrations: 1500 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 500 mg/L

Details on results:

Due to the intensity of colour in the test solution it was not possible to assess symptoms of toxicity during the study.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No mortalities were observed in either the control or the 1500 mg/L test concentration vessel during this study. Therefore, the 96h LC50 was determined to be >1500 mg/L. The No Observed Effect Concentration (NOEC) was determined to be 1500 mg/L. The lowest nominal concentration at which there was 100% mortality was >1500 mg/L.

Executive summary:

Introduction

The study was undertaken to determine the acute toxicity of the test substance to mirror carp Cyprinus carpio. The method was designed to be compatible with the following guidelines:

OECD Guidelines for the Testing of Chemicals (1992). Method 203. Fish, Acute Toxicity Test.

Official Journal of the European Communities, L 383 A, Part C1, Acute Toxicity for Fish (29th December 1992).

Results & Conclusions

During this study there were no mortalities observed in both the dilution water control vessel and the test substance vessel. Therefore the 96h LC50 was determined to be >1500 mg/L. The 96h NOEC was determined to be 1500 mg/L. The lowest nominal concentration at which there was 100% mortality was >1500 mg/L.