Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 July to 28 July, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant guideline study

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

EC Number:

412-780-3

EC Name:

Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

Cas Number:

1380226-46-9

Molecular formula:

A complex mixture of species so no unique molecular formula can be given

IUPAC Name:

dimolybdenum(6+) diammonium 2-{2-[(octadec-9-en-1-yl)amino]ethoxy}ethan-1-ol heptaoxidandiide

Test material form:

liquid: viscous

Details on test material:

- Name of test material (as cited in study report): ADDITIV 104-KONZENTRAT
- Physical state: Liquid (dark red)
- Analytical purity: Product by process; treated as 100% pure
- Lot/batch No.: OS 147
- Expiration date of the lot/batch: March 9, 1994
- Stability under test conditions: Stable (up to 60 degrees C)
- Storage condition of test material: Room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not reported

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Aliquots taken from each of the test concentrations

Test solutions

Vehicle:

no

Details on test solutions:

Weighed amounts of the test substance were spread out on microscope slides. The actual amounts weighed were 49.8, 91.2, 160.7, 280.9 and 501.1 mg. Each slide with test substance was put in 5 liters of ISO-medium. These solutions were stirred for Ca. 67 hours until the start of the exposure. After stirring, the slides were removed. Then the resulting solutions (=Water Accommodated Fractions (WAF's)) were tested, i.e. 2x100ml divided over 2 vessels. The final test solutions ranged from clear to turbid, with test substance particles deposited on the bottom of' the vessel containing the highest test substance concentration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
Species: Daphnia magna (Crustacea, Cladocera) (Straus,1820)
Reason for selection: This system has been selected as an internationally accepted species.
Validity of batch: Frequent inspection of the cultures with respect to number of young, appearance of young and parental daphnia and global feeding
behaviour.
Characteristics: For the test selection of young daphnia with an age of < 24 hours
Start of each batch: With newborn animals, i.e. less than 3 days old.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: Three times a week.
Temperature of medium: 18-22°C, constant within ± 1°C
Feeding: Daily, a suspension of fresh water algae.
Medium: M4, as prescribed by Dr. Elendt—Schneider (Elendt, B.—P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to
antennal damage in Daphnia magna Straus. Protoplasma 154, 25—33).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48

Post exposure observation period:

None

Test conditions

Hardness:

Hardness: 250 mg/l expressed as CaCO3

Test temperature:

The temperature of the medium was measured daily in one control vessel, beginning at the start of the test. The temperature of the test medium measured in the blank control vessel ranged from 18-22°C

pH:

The pH was measured at the beginning and at the end of the test, for all concentrations and the control. The pH ranged from 7.9-8.1

Dissolved oxygen:

The dissolved Oxygen content was measured at the beginning and at the end of the test, for all concentrations and the control. The dissolved Oxygen content ranged from8.2-8.6 mg/l

Salinity:

Not reported

Nominal and measured concentrations:

A range-finding test was performed to provide information about the range of concentrations to be used in the final test. Daphnia were exposed for 48 hours to the WAFs of 10 and 100 mg/l. In the range-finding test 100% immobility of D.magna was recorded in the WAF prepared at 100 mg/l. No immobility was seen in the WAF prepared at 10 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100ml, all glass
- Aeration: No aeration of the test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility at 24 and 48 hours. Daphnia are considered immobile if they do not move after gentle agitation.

TEST CONCENTRATIONS
- Range finding study: Daphnia were exposed for 48 hours to WAFs of 10 and 100 mg/i.
- Test concentrations: WAFs prepared at 10, 18, 32, 56 and 100 mg/i

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

3.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The results indicated that a relative amount of 20% of test substance from each slide had probably dissolved in the water phase of the WAFs. However, analysis of a sample from the WAF prepared at 100 mg/l did not result in a detectable concentration of ADDITIV 104-KONZENTRAT. This was mainly due to the relative low sensitivity of the detection method, especially for samples in other media than THF.

Results with reference substance (positive control):

The responses in the reference test are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was in agreement with the historical data collected at NOTOX. The 48h-EC5O was 0.46 mg/l (95% fiducial limits: 0.35 -0.69 mg/l). The raw data from this study are kept in the NOTOX archives. The test described above was performed under GLP-conditions with a QA-check.

Applicant's summary and conclusion

Conclusions:

Under the conditions of the present study the test substance did not induce acute immobilization of Daphnia magna at WAFs prepared at 18 mg/l or lower. Based on the analytical results, this corresponded with an estimated concentration of 3.6 mg/l.

EC50 after 24 hours of exposure : WAF prepared at 45 mg/l; 95 % fiducial limits: WAFs prepared at 40 and 54 mg/l.
EC50 after 48 hours of exposure: WAF prepared at 34 mg/l; 95 % fiducial limits: WAFs prepared at 25 and 65 mg/l.

Based on the analytical results, the estimated exposure concentrations in the WAFS corresponded with Ca. 20% relative to the prepared concentrations. Hence, the estimated 48h-EC5O was 6.8 mg/l, i.e. corresponding with a WAF prepared at 34 mg/l. Since ADDITIV 104- KONZENTRATwas considered to be insoluble in water and the concentrations in the WAF's were based on indirect measurements, the estimated concentrations have only limited relevance to the actual exposure concentrations.

Executive summary:

In a GLP compliant, guideline 48hr acute toxicity test to Daphnia magna the test substance did not induce acute immobilization of Daphnia magna at WAFs prepared at 18 mg/l or lower. Based on the analytical results, this corresponded with an estimated concentration of 3.6 mg/l.

Based on the analytical results, the estimated exposure concentrations in the WAFS corresponded with Ca. 20% relative to the prepared concentrations. Hence, the estimated 48h—EC50 was 6.8 mg/l, i.e. corresponding with a WAF prepared at 34 mg/l.