Ethanone, 2-chloro-1-(1-chlorocyclopropyl)-

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb- Mar 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

446-620-9

EC Name:

-

Cas Number:

120983-72-4

Molecular formula:

Hill formula: C5H6Cl2O CAS formula: C5H6Cl20

IUPAC Name:

2-chloro-1-(1-chlorocyclopropyl)ethan-1-one

Test material form:

liquid

Details on test material:

purity: 92.3 %

Specific details on test material used for the study:

Purity: 92.1 %
Identity/Stability in the vehicle confirmed analytically.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

5 ml/ kg BW

Doses:

male: 25 and 200 mg/kg bw
female: 25, 200 and 2000 mg/kg bw

No. of animals per sex per dose:

5 anmials per sex and dose

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

> 50 - < 200 mg/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

> 50 - < 200 mg/kg bw

Based on:

test mat.

Mortality:

At 200 mg/kg two males and two females, and at 2,000 mg/kg two females died during the study.

Clinical signs:

other: A dose of 25 mg/kg body weight was tolerated by male and female rats without mortalities and clinical signs. At 200 mg/kg in both sexes piloerection, constipation, decreased motility, decreased reactivity, labored breathing, and in females pallor and unco

Gross pathology:

In animals that died during the observation period the following changes were
detected:
Liver: pale and dark-red discolorations; distinct tabulation;
Lung: slightly collapsed;
Spleen: pale discoloration;
Kidneys: pale discoloration;
Forestomach: thickened; adhesion to abdominal wall;
General observations: autolysis

Animals killed at the end of the study period showed the following changes:
Body cavity: adhesions of stomach (forestomach), and liver to abdominal wall
Liver: pale discoloration; adhesions to forestomach
Stomach: adhesions of forestomach to abdominal wall, forestomach thickened
Spleen: adhesions
Kidneys: pale discoloration

Any other information on results incl. tables

Dose [mg/kg b.w.]	Toxicological results			Duration of signs	Time of death	Mortality [%]
	a)	b)	c)	males
25	0	0	3	-	-	0
200	2	3	3	5h        - 10d	3d        - 6d	67
				females

25	0	0	3	-	-	0
200	2	3	3	5h        - 13d	8d	67
2,000	2	3 -	3	5'        - 14d	2d	67

1. number of dead animals


2. number of animals with signs


3. number of animals in the group

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

LD50: >50 <200 mg/kg body weight

Executive summary:

A study for acute oral toxicity in male and female Wistar rats was conducted with the test substance JAU 6476 - Chloromethylketone.
The method used complied with the OECD - Guideline for Testing of Chemicals No. 423.

A dose of 25 mg/kg body weight was tolerated by male and female rats without mortalities, clinical signs, effects on weight development and gross pathological findings.
At 200 mg/kg in both sexes piloerection, constipation, decreased motility, decreased reactivity, labored breathing, and in females pallor and uncoordinated gait were observed. Additionally to the above described signs, at 2000 mg/kg in females increased motility, increased salivation and in one animal abdominal position were observed. The signs observed occurred within 5 minutes after administration and lasted up to day 14 of the study. 200 mg/kg and 2000 mg/kg were lethal. At 200 mg/kg two males and two females, and at 2000 mg/kg two females died during the study.
The treatment induced a clear body weight depression on day 8 of the study at 200 mg/kg and above. The gross pathology investigations performed at the end of the post-treatment observation period showed pale discolorations on liver, thickened forestomach, pale discolorations on kidneys, and adhesions in the abdominal cavity, where liver, forestomach, spleen and abdominal wall were adhered.