Methanesulfonamide, 1,1,1-trifluoro-N-[(trifluoromethyl)sulfonyl]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 03 Dec 2013 to 14 July 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study, OECD 202 compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

August 2013

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

435-300-4

EC Name:

-

Cas Number:

82113-65-3

Molecular formula:

C2HNO4F6S2

IUPAC Name:

1,1,1-trifluoro-N-trifluoromethanesulfonylmethanesulfonamide

Test material form:

other: aqueous solution

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At exposure initiation (0 hour) and exposure termination (48 hours), a single sample was removed from each test solution and control and analyzed for bistrifluoromethanesulphonimide (TFSIH) concentration. Samples removed at exposure initiation were taken from the intermediate vessels prior to division into replicate test vessels. The samples at exposure termination were a composite of all replicates within each concentration.

Three quality control (QC) samples were also prepared in dilution water at each sampling interval at nominal concentrations which approximated the test concentration range and remained with the exposure solution samples throughout the analytical process. Analysis of the QC samples was used to judge the precision and quality control maintained during the analytical process.

Test solutions

Vehicle:

no

Details on test solutions:

Prior to exposure initiation, a 100 mg/L primary stock solution was prepared by adding 0.4199 g (0.3502 g as active ingredient) of the test substance to 3.5 L of dilution water. The resulting stock solution was mixed for approximately five minutes using a magnetic stir plate and Teflon® coated stir bar and was observed to be clear and colorless with no visible undissolved test substance. Exposure solutions were prepared from the 100 mg/L primary stock solution diluted with the primary stock solution in order to obtained the nominal concentrations of 6.3, 13, 25, 50 and 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: from laboratory cultures maintained at Smithers Viscient
- Age at study initiation (mean and range, SD): < 24 hours old
- Feeding during test: no


ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Food type: unicellular green algae, Ankistrodesmus falcatus ) in addition to a suspension of YCT (yeast, cereal leaves and flaked fish food).
- Amount: 4 × 10E7 cells/mL
- Health during acclimation (any mortality observed): good

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

160 mg/L

Test temperature:

20 - 21°C

pH:

7.2 - 8.1

Dissolved oxygen:

7.5 - 8.7 mg/L

Nominal and measured concentrations:

Nominal: 6.3, 13, 25, 50 and 100 mg/L
Measured concentrations: 6.1, 13, 25, 50 and 97 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250-mL glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: each vessel contained 200 mL of exposure solution
- Aeration: no aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fortified well water
pH: 8.2
Conductivity: 600 µS/cm
Total hardness as CaCO3: 160 mg/L
Total alkalinity as CaCO3: 82 mg/L


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light/8h dark
- Light intensity: 35 to 99 footcandles (380 to 1100 lux)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The pH, dissolved oxygen concentration and temperature were measured at 0, 24 and 48 hours.
The number of immobilized daphnids in each replicate test vessel was recorded at 0, 24 and 48 hours of exposure. Immobilization was defined as the inability to swim within 15 seconds after gentle agitation of the test vessel. Biological observations and observations of the physical characteristics of each replicate exposure solution and control were also made and recorded at 0, 24 and 48 hours, if applicable.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: yes
- Test concentrations: 0.010, 0.10, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: . At exposure termination (48 hours of exposure), no immobilization was observed among daphnids exposed to any of the treatment levels tested. Based on these results and in consultation with the Study Sponsor, a definitive exposure was conducted at nominal concentrations of 6.3, 13, 25, 50 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Several daphnids exposed to the 97 mg/L treatment level were observed to be lethargic at the 24-hour observation period, and all daphnids were observed to be lethargic at exposure termination. At exposure termination (48 hours of exposure), no immobilization was observed among daphnids exposed to any of the treatment levels tested.

Results with reference substance (positive control):

A 24-hour reference test was conducted with daphnids (< 24 hours old) from the laboratory culture. The 24-hour reference test was conducted from 3 to 4 December 2013 and the results established that the 24-hour EC50 value for Daphnia magna and potassium dichromate (Lot No. MKD9039V, tested as 100%) was 2.4 mg/L. This result was within the expected range for daphnids and potassium dichromate.

Any other information on results incl. tables

Table 1: Mean measured concentrations tested, corresponding cumulative percent and number of immobilized organisms, and observations made during the 48-hour static exposure of daphnids (Daphnia magna) to bis trifluoromethanesulphonimide (TFSIH)

Mean Measured Concentration (mg/L)	Percent Immobilization^a
	24-Hour						48-Hour
	A	B	C	D	Mean		A	B	C	D	Mean
Control	0	0	0	0	0		0	0	0	0	0
	(0)	(0)	(0)	(0)			(0)	(0)	(0)	(0)
6.1	0	0	0	0	0		0	0	0	0	0
	(0)	(0)	(0)	(0)			(0)	(0)	(0)	(0)
13	0	0	0	0	0		0	0	0	0	0
	(0)	(0)	(0)	(0)			(0)	(0)	(0)	(0)
25	0	0	0	0	0		0	0	0	0	0
	(0)	(0)	(0)	(0)			(0)	(0)	(0)	(0)
50	0	0	0	0	0		0	0	0	0	0
	(0)	(0)	(0)	(0)			(0)	(0)	(0)	(0)
97	0	0	0	0	0^b		0	0	0	0	0^c
	(0)	(0)	(0)	(0)			(0)	(0)	(0)	(0)

^a The actual number of immobilized daphnids is presented in parentheses.

^b Several daphnids were observed to be lethargic.

^c All daphnids were observed to be lethargic.

Tables 2: The EC₅₀values, corresponding 95% confidence intervals and No‑Observed-Effect Concentration (NOEC) established during the 48‑hour static exposure of daphnids (Daphnia magna) to bis trifluoromethanesulphonimide (TFSIH).

Time Interval	EC50 (mg/L)	95% Confidence Intervals (mg/L)
		Lower	Upper
24 Hour	> 97	NA^a	NA
48 Hour	> 97	NA	NA
NOEC through 48 hours = 50 mg/L
Highest concentration producing 0% immobilization = 97 mg/L
Lowest concentration producing 100% immobilization > 97 mg/L

NA = Not Applicable. EC value was empirically estimated; therefore, 95% confidence intervals could not be determined.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Bis trifluoromethasulfonimide is not harmful to Daphids.

Executive summary:

The purpose of this study was to estimate the acute toxicity (EC₅₀) of bistrifluoromethanesulphonimide (TFSIH) to daphnids (Daphnia magna) under static test conditions, according to the OECD 202 guideline and under GLP conditions.

Nominal concentrations tested were 6.3, 13, 25, 50 and 100 mg/L.

Since no concentration tested resulted in ≥ 50% immobilization, the 48−hour EC50 value for bis trifluoromethanesulphonimide (TFSIH) and Daphnia magna was empirically estimated to be > 100 mg/L, the highest nominal concentration tested. 

The No-Observed-Effect Concentration (NOEC) was determined to be 50 mg/L. 

The highest concentration producing 0% toxicant-related immobilization was 100 mg/L. No concentration tested produced 100% immobilization.