5-thiazolylmethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 April - 29 August, 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted according to OECD method and in accordance with GLP. Study material is well characterized.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

5-thiazolylmethanol

EC Number:

414-780-9

EC Name:

5-thiazolylmethanol

Cas Number:

38585-74-9

Molecular formula:

C4H5NOS

IUPAC Name:

(1,3-thiazol-5-yl)methanol

Method

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium: TA1535, TA1537, TA1538, TA98 and TA100.

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix from Aclor 1254-induced rat liver microsomes

Test concentrations with justification for top dose:

Ten concentrations, 0.01 to 150µl per petri dish were tested for the initial assay and a range of six concentrations, 0.3 -50µl were tested in the independent repeat assay.

Vehicle / solvent:

DMSO

Controls

Controls 1

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Controls 2

Positive controls:

yes

Positive control substance:

other: N-methyl-N-nitro-N-nitrosoguanidine

Controls 3

Positive controls:

yes

Positive control substance:

other: Quinacrine mustard

Controls 4

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Details on test system and experimental conditions:

Petri plates were cultured in an incubator at approximately 37°C for 48hrs

Statistics:

Mean and standard deviation of the triplicate plate counts for each treatment were determined.

Results and discussion

Test results

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

Observations:
Compared with vehicle controls, no concentration related increase in the number of colonies per plate treated with Abbott-87440, with and without activation, were observed.

Toxicity was seen at the highest concentrations.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Under the activated and non-activated conditions, Abbott-87440 was concluded to be non-mutagenic in the bacterial reverse mutation assay.