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EC number: 202-767-9 | CAS number: 99-57-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- no
- Analytical monitoring:
- not required
- Remarks:
- substance is stable under recomended conditions
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg, 500 mg & 1 g of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.42 cm
- Weight at study initiation (mean and range, SD): average 0.117 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 51.2 mg of CaCO3
- Test temperature:
- 24-26 °C
- pH:
- 7.2
- Nominal and measured concentrations:
- 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
8.1. Mortality and symptoms
Nominal conc. (mg/l)
Number of fish
Mortality (cumulated) after
24h
48h
72h
96h
0 (control)
8
-
-
-
-
6.25
8
-
-
-
-
12.5
8
-
-
-
-
25
8
-
-
-
-
50
8
1
-
-
-
100
8
8
-
-
-
Nominal conc. (mg/l)
Number of fish
Symptoms after
24h
48h
72h
96h
0 (control)
8
NS8
NS8
NS8
NS8
6.25
8
NS8
NS8
NS8
NS8
12.5
8
NS8
NS8
NS8
NS8
25
8
NS8
NS8
NS8
NS8
50
8
NS7
NS7
NS7
NS7
Explanation of symptoms:
A: apathy
N: narcotic-like state
C: swimming near water surface
D: swimming at the bottom of bowl
M: Slow movements compared to control
NS: No abnormal symptoms
Number behind symbol for symptom= number of affected fish
8.2. pH and oxygen content:
Nominal conc. (mg/l)
pH readings after
24h
48h
72h
96h
0 (control)
7.6
7.5
7.6
7.7
6.25
7.8
7.5
7.0
6.1
12.5
7.6
7.2
7.1
6.1
25
7.5
7.3
6.9
6.1
50
7.3
7.0
6.5
6.0
Nominal conc. (mg/l)
Oxygen content (mg/l) after
24h
48h
72h
96h
0 (control)
6.79
6.72
6.69
6.68
6.25
6.76
6.73
6.10
6.09
12.5
6.47
6.21
6.01
5.93
25
6.14
6.01
5.99
5.71
50
6.05
6.01
5.77
5.67
8.3. Test temperature
Nominal conc. (mg/l)
Temperature (°C) after
24h
48h
72h
96h
0 (control)
25
26
26
26
6.25
25
26
26
26
12.5
25
26
26
26
25
25
26
26
26
50
25
26
26
26
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be >50 mg/l.
- Executive summary:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg, 500 mg & 1 g of the test substance in 10 liters of potable water passed through reverse osmosis system with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >50 mg/l LC100 was observed at the concentration 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic to fish and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Reference
Description of key information
Short term toxicity to fish:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg, 500 mg & 1 g of the test substance in 10 liters of potable water passed through reverse osmosis system with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >50 mg/l LC100 was observed at the concentration 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic to fish and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 50 mg/L
Additional information
Short term toxicity to fish:
The toxicity of test material for fish was evaluated using data from lab report along with a publication.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg, 500 mg & 1 g of the test substance in 10 liters of potable water passed through reverse osmosis system with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >50 mg/l LC100 was observed at the concentration 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic to fish and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
In a peer reviewed journal ,short term toxicity to fish was evaluated for the test chemical for 48 h . Carp(Ciprinus carpio) and was provided by Lu-Kan Fish Culture Station, Taiwan Fisheries Research Institute, Taichung, Taiwan. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests was performed. Four carp , 2 to 6 cm in size, were introduced to each 10-L beaker containing5 L of different concentrations of test chemical . Five different concentrations 0.1 , 0.5 , 1 , 2 and 4 mg/l were prepared. Sub-lethal concentration (LC50) values were calculated by the trimmed Spearman} act concentration EC50 of test material on fish when observed after 48 h was noted to be 0.453 mg/l. Based on the above effect concentration it can be concluded that the test material is highly toxic to fish and can be classified as aquatic acute 1.
As we have two studies which are classifying test material in two diffrent categories , hence we will consider effect concentration obtained from lab report as it has higher relaibility then that of data from publication .Based on the LC50, it can be consider that the chemical was toxic to fish and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
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