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EC number: 468-880-2 | CAS number: 102985-93-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial radical formation potential
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-08-27 to 2020-10-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on preparation and application of test substrate:
- The constituents of artificial soil (calculated as dry weight) are listed as follows:
-10 % sphagnum peat (no visible plant remains, finely ground, dried and the moisture content was measured, calculated as dry weight);
- 20 % kaolin clay
- 70 % quartz sand.
The artificial soil (calculated as dry weight) was prepared and mixed thoroughly in a mixer. The pH value of the soil was determined to be 6.28. The dry artificial soil was taken and add pure water to premoisten it. Before the test, the wet soil was sampled, four replicates, and dried for about 3 hours at 105 °C to determine the moisture content to be 13.3 %. - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm
- Source: Wuxi Earthworm Breeding Base
- Age at test initiation: Earthworms were adults (at least two months old with clitellum)
- Weight at test initiation: 332 – 492 mg
ACCLIMATION
Prior to test, the earthworms were placed in the artificial soil 24 hours under the test condition. - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 19.1 – 21.7 °C
- pH:
- 6.51 – 6.58
- Moisture:
- Control groups: 32.0 % - 32.8 %;
Test item treatment group: 31.5 % - 32.0 % - Details on test conditions:
- TEST SYSTEM
- Amount of soil or substrate: 750 g dw
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per solvent control: 4
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 400 – 800 Lux
EFFECT PARAMETERS MEASURED: The survivals and toxic symptoms of the test earthworms were assessed after 7 and 14 days of exposure
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 20, 100, 500 and 1000 mg/kg
- Results used to determine the conditions for the definitive study: yes - Nominal and measured concentrations:
- Nominal conc.: 1000 mg/kg
Measure conc.: not determined - Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Therefore, the 7d-LC50 and 14d-LC50 of test item to earthworm (Eisenia fetida) were more than 1000 mg/kg.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, the 7d-LC50 and 14d-LC50 of test item to earthworm (Eisenia fetida) were more than 1000 mg/kg.
- Executive summary:
The study was performed to evaluate the acute toxicity of test item to earthworm (Eisenia fetida). The artificial soil was selected as test medium, the test duration was 14 days. The adult earthworms were exposed to the artificial soil with test item concentrations of 1000 mg/kg. Blank control and solvent control were set in parallel. Four replicates were prepared for each group and each replicate contained 10 earthworms. The survivals and toxic symptoms of the test earthworms were assessed after 7 and 14 days of exposure. The LC50 and its 95% confidence limits of test item to earthworm were calculated. The mean wet weight of the earthworms in control groups and test item treatment group was 312 – 394 mg at the start of the test. The mean wet weight of the earthworms was 300 – 386 mg at the end of the test. After 7 days and 14 days, the mortality of earthworms in blank control group was 0 %, and there were no abnormal symptoms. After 7 days and 14 days, the mortality of earthworms in solvent control treatment group and test item treatment group was 2.5 % and there were no abnormal symptoms. In this study, the 7d-LC50 and 14d-LC50 of test item to earthworm (Eisenia fetida) were more than 1000 mg/kg.
Reference
Validity of the Test
The mortality rate of earthworms was 0% in the blank control group and 2.5% in the solvent control group at the end of the test (less than 10%), which satisfied the validity of the test.
Results and Discussion
After 7 days and 14 days, the mortality of earthworms in blank control group was 0%, and there were no abnormal symptoms. After 7 days and 14 days, the mortality of earthworms in solvent control treatment group and test item treatment group was 2.5 % and there were no abnormal symptoms.
The mean wet weight of the earthworms in control groups and test item treatment group was 312-394 mg at the start of the test. The mean wet weight of the earthworms was 300-386 mg at the end of the test.
Table 1: Mortality of Earthworm
Concentration (mg/kg) | Replicates | Number of initial exposed earthworms was 10 for each replicate | |||||
Cumulative number of death | Cumulative Mortality(%) | Abnormal Symptoms | |||||
7-d | 14-d | 7-d | 14-d | 7-d | 14-d | ||
Blank Control | 1 | 0 | 0 | 0 | 0 | / | / |
2 | 0 | 0 | |||||
3 | 0 | 0 | |||||
4 | 0 | 0 | |||||
Solvent Control | 1 | 0 | 0 | 2.5 | 2.5 | / | / |
2 | 0 | 0 | |||||
3 | 1 | 1 | |||||
4 | 0 | 0 | |||||
1000 | 1 | 0 | 0 | 2.5 | 2.5 | / | / |
2 | 1 | 1 | |||||
3 | 0 | 0 | |||||
4 | 0 | 0 |
Remark: /" means the survived earthworms have no abnormal symptoms.
Table 2: Weight of Earthworm
Concentration (mg/kg) | Replicate | Earthworms total weight (mg) | Mean Weight (mg) | ||
0-d | 14-d | 0-d | 14-d | ||
Blank Control | 1 | 3936 | 3858 | 394 | 386 |
2 | 3929 | 3711 | 393 | 371 | |
3 | 3744 | 3590 | 374 | 359 | |
4 | 3910 | 3803 | 391 | 380 | |
Solvent Control | 1 | 3549 | 3400 | 355 | 340 |
2 | 3555 | 3367 | 356 | 337 | |
3 | 3306 | 2754 | 331 | 306 | |
4 | 3696 | 3485 | 370 | 349 | |
1000 | 1 | 3124 | 2999 | 312 | 300 |
2 | 3829 | 3360 | 383 | 373 | |
3 | 3418 | 3351 | 342 | 335 | |
4 | 3719 | 3598 | 372 | 360 |
Description of key information
The 7d-LC50 and 14d-LC50 of test item to earthworm (Eisenia fetida) were more than 1000 mg/kg.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
The study was performed to evaluate the acute toxicity of test item to earthworm (Eisenia fetida). The artificial soil was selected as test medium, the test duration was 14 days. The adult earthworms were exposed to the artificial soil with test item concentrations of 1000 mg/kg. Blank control and solvent control were set in parallel. Four replicates were prepared for each group and each replicate contained 10 earthworms. The survivals and toxic symptoms of the test earthworms were assessed after 7 and 14 days of exposure. The LC50 and its 95% confidence limits of test item to earthworm were calculated. The mean wet weight of the earthworms in control groups and test item treatment group was 312 – 394 mg at the start of the test. The mean wet weight of the earthworms was 300 – 386 mg at the end of the test. After 7 days and 14 days, the mortality of earthworms in blank control group was 0 %, and there were no abnormal symptoms. After 7 days and 14 days, the mortality of earthworms in solvent control treatment group and test item treatment group was 2.5 % and there were no abnormal symptoms. In this study, the 7d-LC50 and 14d-LC50 of test item to earthworm (Eisenia fetida) were more than 1000 mg/kg.
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