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Environmental fate & pathways

Adsorption / desorption

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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jun 2006 to Feb 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
Sponsor batch no: 151105Purity: 99.9%
Radiolabelling:
not specified

Study design

Test temperature:
Column temp 25C

HPLC method

Details on study design: HPLC method:
The distribution coefficient was estimated by HPLC simulation method using isocratic elution. The pr ocedue conformed to those outlined EC Directive 92/69/EEC Method C19 and OECD Guideline 121. The following HPLC system was used:-Column: 250 x 4.6 mm Zorbax CNMobile phase: Methanol / Water 55/45 v/vInjection volume: 20uL Column temp 25CFlow rate: 1ml/minDetection wavelength: 230 nmPeak response: Area Cycle time: Typically 20 mins

Batch equilibrium or other method

Analytical monitoring:
yes

Results and discussion

Adsorption coefficient
Type:
Koc
Value:
ca. 3.68

Results: HPLC method

Details on results (HPLC method):
The soil adsorption coefficient of Beta Ketosulfide was evaluated using the HPLC simulation technique with a mobile phase of methanol /water, 55/45 v/v. The test substance eluted as a single component with a retention time corresponding to a log Koc value of +3.68, within a mean 95% confidence range of +3.12 to +5.00.

Any other information on results incl. tables

The test substance, Beta Ketosulfide eluted as asingle peak with a retention time within the rangeof retention times of the calibration substances.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance Beta Ketosulfide eluted as a single peak with a retention time within the range of retention times of the calibration substances. Therefore the results of the estimation may be expected to be realistic, as the results were calculated b interpolation rather than extrapolation. It was possible to establish the calibration line with six different calibration compounds, five eluting before the test substance and one eluting after it.There were only minimal differences between the replicated of retention times for each of the ca libration and test substances, and between the two injection sequences. The resultant calculated va lues differed only to a small extent, therefore the results are considered acceptable for regulatory purposes.The distribution coefficient of Beta Ketosulfide was successfully evaluated using the HPLC simu lation technique, with a mobile phase of methanol/water, 55:45 v/v. The test substance eluted as a single component exhibiting a retention time corresponding to a log Koc value of +3.68, within a 95% confidence range of +3.12 to +5.00
Executive summary:

The substance was tested in accordance with EC Directive 92/69/EEC Method C19 and OECD guideline 121. The soil adsorption coefficient of Beta Ketosulfide was evaluated using the HPLC simulation technique with a mobile phase of methanol/water, 55/45 v/v. The test substance eluted as a single component with a retention time corresponding to a log Koc value of +3.68, within a mean 95% confidence range of +3.12 to +5.00.