Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 469-300-0 | CAS number: 63675-73-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jul 2006 to Feb 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 469-300-0
- EC Name:
- -
- Cas Number:
- 63675-73-0
- Molecular formula:
- Hill Empirical Formula: C16H16O3S CAS Empirical Formula: C16H16O3S
- IUPAC Name:
- 1-(4-methoxyphenyl)-2-[(3-methoxyphenyl)sulfanyl]ethan-1-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- Lot no: 151105Purity - 101.39%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- A sample of activated sludge was collected from one of the return lines at Burley Menston sewage treatment works (West Yorkshire, UK), a treatment works with a waste-water catchment that is predominantly domestic. On arrival at the laboratory, the sample was aerated by means of a compressed air supply delivered through a diffuser block.
- Duration of test (contact time):
- 56 d
Initial test substance concentration
- Initial conc.:
- 15 mg/L
- Based on:
- IC (inorganic carbon)
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Four treatment groups were established:Controlinoculated mineral salts mediumReferenceinoculated mineral salts medium plus sodium benzoate at 15 mgC/LTestinoculated mineral salts medium plus test substance at 15 mgC/LToxicity controlinoculated mineral salts medium plus both the test and reference substances each at 15 mgC/L.The purpose of the toxicity control was to assess the biodegradation of the reference substance in the presence of the test substance.Duplicate vessels were established in the test, reference and control groups. A single vessel was established for the toxicity control.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 1 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 2 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 3 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 5 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 8 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 11 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 18 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 22 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 26 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 27 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 16
- Sampling time:
- 32 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 21
- Sampling time:
- 35 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 26
- Sampling time:
- 39 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 28
- Sampling time:
- 42 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 30
- Sampling time:
- 46 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 31
- Sampling time:
- 49 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 32
- Sampling time:
- 53 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 33
- Sampling time:
- 56 d
BOD5 / COD results
- Results with reference substance:
- See below for results of test substance compared to reference and toxicity control
Any other information on results incl. tables
Overall results
% Biodegradation | ||||||||||
Days | 1 | 2 | 3 | 5 | 8 | 11 | 14 | 18 | 22 | 26 |
Test * | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Reference** | 16 | 34 | 43 | 53 | 62 | 68 | 74 | 79 | 84 | 87 |
Toxicity Control *** (% total ThCO2) | 10 | 20 | 25 | 28 | 31 | 32 | 34 | 35 | 38 | 44 |
Toxicity Control *** (% sodium benzoate ThCO2) | 19 | 40 | 49 | 57 | 61 | 64 | 67 | 71 | 76 | 88 |
% Biodegradability | ||||||||||
Day | 27 | 28 | 32 | 35 | 39 | 42 | 46 | 49 | 53 | 56 |
Test* | 1 | 6 | 16 | 21 | 26 | 28 | 30 | 31 | 32 | 33 |
Reference** | 88 | 89 | 90 | 91 | 92 | 92 | 93 | 94 | 94 | 96 |
Toxicity control*** (% total ThCO2) | 48 | 50 | 54 | 56 | 59 | 61 | 62 | 63 | 64 | 66 |
Toxicity control *** (% sodium benzoate ThCO2) | 95 | 100 | 107 | 113 | 118 | 121 | 124 | 126 | 128 | 133 |
* = Beta ketosulfide (15 mgC/L)
** = Sodium Benzoate (15mgC/L)
***= Beta Ketosulfide (15 mgC/L) plus Sodium Benzoate (15mgC/L)
Beta ketosulfide
The test substance showed no evident of biodegradation until Day 28 of the test, when a sharp increase in the rate of CO2 evolution from the test vessels was noted. The duration of the test was extended beyond 28 days. Biodegradation of the test substance was considered to have attained a plateau by Day 56. The final mean % degradation for vessels containing Beta Ketosulfide was 33%. To be considered to be readily biodegradable,a test substance must achieve 60% biodegradation by the end of the test. Additionally, the rate of biodegradation must be sufficient to reach 60% biodegradation within ten days of reaching 10%. Consequently, Beta Ketosulfide cannot be considered to be readily biodegradable.
Sodium benzoate
Rapid biodegradation commenced immediately and declined to a more gradual rate after approximately 8 days. Biodegradation had achieved 60% in both vessels by day 8. Mean biodegradation in the reference vessels was 73% on day 14, 89% on day 28 and 96% at the end of the test.
Toxicity control
Vigorous CO2 production began immediately in the toxicity control. The rate of CO2 production generally followed that observed in the two reference vessels containing sodium bezoate alone but at a slightly reduced absolute amount. The onset of biodegradation of the test substance in the vessel is evident from day 28 onwards where a sharp increase in CO2 evolution is noted as in the test vessels.
Biodegradation of sodium benzoate in the vessel, based on the theoretical yield of the reference and test substances, achieved 34% on Day 14 of the test. The calculated result for biodegradation in this vessel was 66% on Day 56. Biodegradation of sodium benzoate in the vessel, based on the teoretical yield of the reference substance alone, achieved 67% on Day 14 of the test. The calculated result for biodegradation in this vessel was 100% on Day 28 and exceeded 100% thereafter. This is because the calculation is based upon the maximwn theoretical amount of carbon dioxide evolution possible through biodegradation of the reference substance alone. Consequently, the calculation does not make allowance for the contribution to the carbon dioxide evolution in this vessel resulting from biodegradation of the test substance. The absence of any significant suppression ofbiodegradation when compared to the
performance of sodium benzoate alone demonstrates that the Beta Ketosulfide present was not toxic to the test system. This is further supported by the level of biodegradation of the test substance observed.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Beta Ketosulfide cannot be considered to be readily biodegradable. Beta Ketosulfide is considered to have shown significant evidence of mineralisation over 56 days.
- Executive summary:
The study was carried out in accordance with the method described in Annex V of EU Directive 67/548/ EEC: Part C4: Biodegradation: determination of 'ready' biodegradability, method C - Carbon Dioxide Evolution test.The method is identical to the1992 revision of OECD Guideline 301B and that in section (m) of US EPA OPPTS method 835.3110. The test was extended beyond the standard 28 day incubation period on the basis of the results obtained.
After a lag period of 28days, biodegradation of Beta Ketosulfide attained a plateau by Day 56.The final mean % biodegradation value for vessels containing Beta Ketosulfide was 33%.
As 60% biodegradation of Beta Ketosulfide was not achieved by the end of the test,Beta ketosulfide cannot be considered to be readily biodegradable
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.