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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 625-Triketon
- Physical state: solid, beige
- Analytical purity: 94%
- Batch No.: LJ 27881/1

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: was added directly

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Initial biomass concentration: 1g/L

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
20 ± 2 °C
Nominal and measured concentrations:
Nominal test concentration: 20, 100, 250, 500, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 ml fill volume, 8 ml/ vessel synthetic medium (OECD medium)
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: 1g /L

Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 630 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
Effect concentrations (30 min):
EC20 = < 1mg/L
EC50 = 10 mg/L
EC80 = ca. 62 mg/L

Applicant's summary and conclusion