Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 625 - Triketone
- Physical state: solid
- Analytical purity: 94%
- Impurities (identity and concentrations): diketone, water
- Lot/batch No.: LJ 27 881/1

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- The following nominal concentrations were analysed: control, 1.56, 12.5, 100 mg/L
- At the start of the test (after 0 h) samples from vessels which were not stocked with daphnids were analysed and at the end of the test (after 48 h) samples from stocked and unstocked vessels were analysed.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: At the beginning of the test the stock solution of the test substance was freshly prepared. A defined amount of the substance was weighed out and suspended directly in the test medium and stirred for about 20 h at 20 °C. Afterwards small amounts of undissolved particles were removed by means of centrifugation (20 min. at about 7000G). The test was performed with the supernantant.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: watre flea
- Source: supplied by Institut National de Recherche Chimique Appliquée, France, in 1978 and cultured under standard contiotions in the laboratory
- Age at study initiation: 2- 24 h
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.47 mmol/L
Test temperature:
19.8 - 21.3 °C
pH:
7.9 - 8.0
Dissolved oxygen:
8.2 - 8.5
Nominal and measured concentrations:
Nominal test concentrations: control, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: test tubes (glass) with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium M4 is used for the culture and test. The medium is prepared on the basis of an ultrapure, deionized water (conductivity 0.05 µS/cm).
- Alkalinity: 0.84 mmol/L up to pH 4.3
- Conductivity: 636 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: after 0, 24,48 h in an extra vessel close to the test vessel, After 0 and 48 h in one replicate at each concentration

OTHER TEST CONDITIONS
- Photoperiod: day : night-rhythm 16 : 8 h
- Light intensity: about 5 -6 µE/(m²*s) in the range of 400-700 nm

EFFECT PARAMETERS MEASURED: swimming ability after 0, 3, 6, 24, 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
31.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 27.4 -36-7 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: none

Applicant's summary and conclusion