Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dimethylbenzylidenecyclopentanone 95 %
- Lot/batch No.: COD-000462
- Analytical purity: 96.6 %

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added to deionized water and the mixture was stirred for 22±2 hours. Subsequently synthetic medium was added.
added and the aeration was started.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge taken from a municipal wastewater treatment plant of the city Mannheim, Germany
- Initial biomass concentration: 1.0 g/l

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
180 min

Test conditions

Test temperature:
20 ± 2 °C
pH:
7.0 - 7.7
Nominal and measured concentrations:
Nominal test substance concentration:
999 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 mL/vessel 100-fold concentrated OECD medium
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenole

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 999 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 999 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC20 (180 min)ca. 3.5 mg/l

Applicant's summary and conclusion