(E)-5[(4-chlorophenyl)methylene]-2,2-dimethylcyclopentanone

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

131984-21-9

Cas Number:

131984-21-9

IUPAC Name:

131984-21-9

Details on test material:

- Name of test material (as cited in study report): Dimethylbenzylidenecyclopentanone 95 %
- Lot/batch No.: COD-000462
- Analytical purity: 96.6 %

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added to deionized water and the mixture was stirred for 22±2 hours. Subsequently synthetic medium was added.
added and the aeration was started.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge taken from a municipal wastewater treatment plant of the city Mannheim, Germany
- Initial biomass concentration: 1.0 g/l

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

180 min

Test conditions

Test temperature:

20 ± 2 °C

pH:

7.0 - 7.7

Nominal and measured concentrations:

Nominal test substance concentration:
999 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 mL/vessel 100-fold concentrated OECD medium

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenole

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 999 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 999 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20 (180 min)ca. 3.5 mg/l