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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
131984-21-9
Cas Number:
131984-21-9
IUPAC Name:
131984-21-9
Details on test material:
- Name of test material (as cited in study report): RPA 405217
- Lot/batch No.: DA 165

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by diluting 0.32 g test substance/l with the help of Tween 80. The test solutions were prepared separately.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.1 g/l

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: No. 86.81 SAG
- Source (laboratory, culture collection): algae culture of the University of Göttingen.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22.5 ± 1 °C
pH:
8.8 - 9.3
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
5, 10, 20, 40, 80 and 160
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Fill volume: 250 ml
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline OECD 201


OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: 8000 lux (0.72 x E20 photons/m²s)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
3.44 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
35.41 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
364.49 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
Self fluorescence of the test substance: 0.01 in the highest test concentration

Applicant's summary and conclusion