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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dimethylbenzylidenedyclopentanone 95 %
- Lot/batch No.: COD-000462
- Analytical purity: 96.6 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentrations were prepared separately by stirring the test substance in M4 medium for 20 hours.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age of parental stock (mean and range, SD): 2-24 h
- Food type: Desmodesmus subspicatus
- Amount: 1.54 ml/400 ml
- Frequency: once per day

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
21 d

Test conditions

Hardness:
2.20 - 3.20 mmol/I
Test temperature:
19.9 - 20.7 °C
pH:
7.9 - 8.2
Dissolved oxygen:
8.8 - 9.8 mg/l
Nominal and measured concentrations:
Nominal test concentration: 2.5 mg/l

Mean analytical determined concentration: 0.093 mg/l (2.0 - 6.4 % of nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: glass cups with a bottom of stainless steel grid placed in glass beakers (600 ml)
- Fill volume: 200 ml
- Renewal rate of test solution (frequency/flow rate): each Monday, Wednesday and Friday
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: ca. 0.02 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to OECD 211
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: artificial light, type warm white (OSRAM L58 W31)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Reproduction, weight and length of test animals.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 0.093 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
The analytically determined test concentration of 0.093 mg/L was still above the limit of water solubility of the substance.
Results with reference substance (positive control):
EC50(24h): 0.64 mg/I
Reported statistics and error estimates:
Student's t-est (one-sided, p:9 0,05; p:9 0,01)

Any other information on results incl. tables

Reproduction

NOEC >= 2.5 mg/l (nominal), >=0.093 mg/l (measured)

LOEC > 2.5 mg/l (nominal), 0.093 mg/l (measured)

LC0 >= 2.5 mg/l (nominal),

0.093 mg/l (measured)

Length (NOECILOEC) of the parent animals

NOEC >= 2.5 mg/l (nominal), >=0.093 mg/l (measured)

LOEC > 2.5 mg/l (nominal), 0.093 mg/l (measured)

Weight (NOEC/LOEC) of the parent animals

NOEC >= 2.5 mg/l (nominal), >=0.093 mg/l (measured)

LOEC > 2.5 mg/l (nominal), 0.093 mg/l (measured)

Applicant's summary and conclusion

Conclusions:
No toxic effects were observed within the range of water solubility.