Registration Dossier
Registration Dossier
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EC number: 410-440-9 | CAS number: 164058-20-2
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (E)-5[(4-chlorophenyl)methylene]-2,2-dimethylcyclopentanone
- EC Number:
- 410-440-9
- EC Name:
- (E)-5[(4-chlorophenyl)methylene]-2,2-dimethylcyclopentanone
- Cas Number:
- 164058-20-2
- Molecular formula:
- C14 H15 Cl O
- IUPAC Name:
- (5E)-5-[(4-chlorophenyl)methylidene]-2,2-dimethylcyclopentan-1-one
- Details on test material:
- - Name of test material (as cited in study report): Dimethylbenzylidenedyclopentanone 95 %
- Lot/batch No.: COD-000462
- Analytical purity: 96.6 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentrations were prepared separately by stirring the test substance in M4 medium for 20 hours.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age of parental stock (mean and range, SD): 2-24 h
- Food type: Desmodesmus subspicatus
- Amount: 1.54 ml/400 ml
- Frequency: once per day
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 2.20 - 3.20 mmol/I
- Test temperature:
- 19.9 - 20.7 °C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 8.8 - 9.8 mg/l
- Nominal and measured concentrations:
- Nominal test concentration: 2.5 mg/l
Mean analytical determined concentration: 0.093 mg/l (2.0 - 6.4 % of nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass cups with a bottom of stainless steel grid placed in glass beakers (600 ml)
- Fill volume: 200 ml
- Renewal rate of test solution (frequency/flow rate): each Monday, Wednesday and Friday
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: ca. 0.02 animals/ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to OECD 211
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: artificial light, type warm white (OSRAM L58 W31)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Reproduction, weight and length of test animals. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.093 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- The analytically determined test concentration of 0.093 mg/L was still above the limit of water solubility of the substance.
- Results with reference substance (positive control):
- EC50(24h): 0.64 mg/I
- Reported statistics and error estimates:
- Student's t-est (one-sided, p:9 0,05; p:9 0,01)
Any other information on results incl. tables
Reproduction
NOEC >= 2.5 mg/l (nominal), >=0.093 mg/l (measured)
LOEC > 2.5 mg/l (nominal), 0.093 mg/l (measured)
LC0 >= 2.5 mg/l (nominal),
0.093 mg/l (measured)
Length (NOECILOEC) of the parent animals
NOEC >= 2.5 mg/l (nominal), >=0.093 mg/l (measured)
LOEC > 2.5 mg/l (nominal), 0.093 mg/l (measured)
Weight (NOEC/LOEC) of the parent animals
NOEC >= 2.5 mg/l (nominal), >=0.093 mg/l (measured)
LOEC > 2.5 mg/l (nominal), 0.093 mg/l (measured)
Applicant's summary and conclusion
- Conclusions:
- No toxic effects were observed within the range of water solubility.
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