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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

Daphnia magna was exposed under static conditions for 48 h to one concentration (100 mg/L) of the test substance. No toxic effect was observed.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

A study was performed to assess the acute toxicity of the substance to Daphnia magna STRAUS under static conditions. The study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004). The Daphnia were exposed to a limit test concentration of nominally 100 mg/L of the substance dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax, a magnetic stirrer and a folded filter. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure.

No toxic effects against Daphnia were observed at a limit effective loading of 100 mg/L. The results are expressed in terms of Effective Loadings (EL). As the substance is a multi-constituent substance and only poorly soluble in water, a suitable selective and sensitive chromatographically method for the determination of the substance in aqueous solutions could not be established. Therefore a Water Accommodated Fraction (WAF) was used to test effects at a limit effective loading of 100 mg/L, and no specific analysis was performed. The content of the test item during the exposure period was verified by DOC determination.