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EC number: 700-910-4 | CAS number: 1354201-99-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 September - 6 October 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Also in accordance with GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- O,O-bis(2-methylpropyl) sulfanidylphosphonothioate; trimethylazanium
- EC Number:
- 700-910-4
- Cas Number:
- 1354201-99-2
- Molecular formula:
- C11H28NO2PS2
- IUPAC Name:
- O,O-bis(2-methylpropyl) sulfanidylphosphonothioate; trimethylazanium
- Test material form:
- solid
- Remarks:
- pale yellow glassy solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): S-10713
- Substance type: Straw color, lump solid
- Physical state: solid
- Lot/batch No.: AQ11216TZ
- Expiration date of the lot/batch: July 2013
- Stability: Stable under normal conditions
- Storage condition of test material: Room temperature; in the dark
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations (nominal):
Range finding test - Control, 1, 10, 100 and 1000 mg/L
Definitive tests:
Nr. 1: 0, 10, 20, 40, 80, 160 and 320 mg/L
Nr. 2: 0, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000 and 2000 mg/L
- Sampling method:
For the range-finding and definitive tests, samples were collected from pooled control and exposure solutions at the beginning and end of the test in 20 mL vials (pre-cleaned glass with Teflon lined caps) provided by Investigative Science Incorporated laboratory (ISI - 1050 Cooke Boulevard, Unit 2 Burlington, Ontario L7T 4A8 Canada) and pre-charged with a preservative (nitric acid). The vials were filled, leaving some headspace. A single set of samples were collected from the range-finding test and duplicate samples were collected from the definitive tests. One set of the duplicate samples was retained by AquaTox for use as backup in the event that a sample was lost, damaged, or if re-analysis was required.
- Sample storage conditions before analysis:
The 0-hour and 48-hour samples were stored in the dark at 4 ± 2°C prior to being picked up for analysis. The samples were kept cool (packed in a cooler with an ice pack) while being delivered to the analytical laboratories for analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - All S-10713 test solutions for the range-finding and definitive test were prepared on the basis of the whole product concentration (i.e., without correction for purity) and without the use of any solubilizing agents.
- Test concentrations for the range-finding test were based on a logarithmic series and included nominal S-10713 concentrations of 1, 10, 100 and 1000 mg/L plus a dilution water control (100% laboratory water). The solution containing the highest nominal S-10713 concentration of 1000 mg/L was prepared by weighing out 0.5003 g of the test item into a 500 mL volumetric flask and making this up to a 500 mL volume using dilution water. Gentle stirring was applied for approximately 1. 5 hours. All lower concentrations were prepared using a ten-fold serial dilution beginning with the 1000 mg/L test solution.
- Test concentrations for the definitive tests were based on a geometric series. The initial test included nominal S-10713 concentrations of 10, 20, 40, 80, 160 and 320 mg/L plus a dilution water control (i.e., 100% laboratory water). The solution containing the highest nominal S-10713 concentration of 320 mg/L was prepared by weighing out 0.3201 g of the test item into a 1 L volumetric flask and making this up to a 1000 mL volume using dilution water. Gentle stirring was applied for approximately 1. 5 hours. All lower concentrations were prepared by serial dilution, using a 0.5 times dilution factor, beginning with the 320 mg/L test solution.
- The second definitive test was conducted using nominal S-10713 concentrations of 15.6, 31.3, 62.5, 125, 250, 500, 1000, and 2000 mg/L. The 1000 and 2000 mg/L solutions were prepared individually, followed by gentle stirring for approximately 1. 5 hours. The 2000 mg/L solution was prepared by weighing 1.0005 g of S-10713 into a 500 mL volumetric flash and making this up to a 500 mL volume using laboratory dilution water. The 1000 mg/L solution was prepared by weighing 1.0009 g of S-10713 into a 1 L volumetric flash and making this up to a 1000 mL volume using laboratory dilution water. All lower concentrations were prepared by serial dilution, using a 0.5 times dilution factor, beginning with the 1000 mg/L solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Source: The organisms were originally obtained from the Ministry of the Environment (Toronto, Ontario, Canada). The population has been cultured continuously in AquaTox’s laboratory since 1988.
- Age at study initiation: First instar, neonate (<24 h old)
- Feeding during test: None
ACCLIMATION
- Acclimation conditions (same as test or not): yes
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- ~ 200 mg/L as CaCO3
- Test temperature:
- 20 ± 2 °C
- pH:
- Definitive test Nr. 2 :
- Initial: 7.5-8.4
- Final: 8.0-8.5 - Dissolved oxygen:
- Definitive test Nr. 2 (mg/L) :
- Initial: 8.6-8.5
- Final: 8.2-8.8 - Nominal and measured concentrations:
- - Results were calculated using nominal concentrations since the difference between nominal and measured values was <20%.
Please see data provided in point: "Any other information on results incl. tables". - Details on test conditions:
- TEST SYSTEM (definitive test Nr. 2)
- Test vessel: Glass beaker
- Material, size, headspace, fill volume: Glass, with a capacity of 250 ml and filled with 100 ml of test solution
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Loading rate: Approximately 20 mL/daphnid
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
The source of water used for D. magna culturing and testing was moderately hard groundwater (~300 mg/L as CaCO3) obtained from an aquifer in Aberfoyle, Ontario, Canada. For culture and testing purposes with D. magna, the groundwater was filtered through a 20 micron cellulose-acetate filter and diluted with Reverse Osmosis water to achieve a hardness of ~200 mg/L (as CaCO3).
A continuous supply of oil-free compressed air was provided to bring the pH and concentrations of dissolved oxygen and other gases into equilibrium with air. The concentration of dissolved oxygen in the water was maintained at >80% of the air saturation value.
- Culture medium different from test medium: No
- Intervals of water quality measurement:
Dissolved oxygen, pH, conductivity at start and end of test, temperature and visual observations at 0, 24 and 48 hours, dilution water hardness at 0 hours.
OTHER TEST CONDITIONS
- Photoperiod: 16-hour light, 8-hour dark (with 30 minute transition period)
- Light intensity: 400 to 800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
Range finding test: 10
Definitive test:
Nr. 1: 2
Nr. 2: 2
- Range finding study
- Test concentrations: control, 1, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study yes - Reference substance (positive control):
- yes
- Remarks:
- Sodium chloride
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 121 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 85-171
- Details on results:
- - Complete immobility of D. magna (i.e., 100%) was observed within the first 24 hours at nominal S-10713 concentrations of 1000 and 2000 mg/L, with partial immobility at the 500 and 250 mg/L concentrations of 55% and 5% respectively. No immobility of daphnids was observed at lower concentrations within the first 24 hours. At test termination, partial immobility of daphnids (20% to 80%) was observed at intermediate exposure concentrations from 5.6 mg/L to 500 mg/L, with complete immobility at higher concentrations (≥ 1000 mg/L) and no immobility at lower concentrations (≤ 7.8 mg/L).
- Results with reference substance (positive control):
- The 48-hour LC50 for the Daphnia magna reference toxicant (sodium chloride) test was 6.0 mg/L (95% CL: 5.4 - 6.4 mg/L)
- Reported statistics and error estimates:
- - The CETIS© (2007) program was used to calculate the 24- and 48-hour EC50s (and 95% Confidence Limits) according to the methods of Linear Regression and Spearman-Karber, respectively.
Any other information on results incl. tables
Table 1. Test substance (S-10713) water concentrations in the range-finding test.
Nominal Concentration (mg/L) |
Measured Concentration (mg/L) |
Measured Concentration as a Percentage of Nominal (%) |
||
0 hour |
48 hour |
0 hour |
48 hour |
|
1000 |
1026 |
1089 |
102.6 |
108.9 |
100 |
101.9a |
108.2 |
101.9 |
108.2 |
10 |
8.9 |
9.5 |
89 |
95 |
1 |
0.9 |
1.0 |
90 |
100 |
Control |
0 |
<MDLb |
N/Ac |
N/A |
a Average
of duplicate analysis. RPD = 1.2%
b Method Detection Limit =
0.7 mg/L
c Not Applicable
Table 2. Summary of the Definitive Test Results of test # 2. Including Measured Exposure Concentrations of S-10713 (after 0 and 48 hours) and Immobility of D. magna (after 24 and 48 hours). | ||||||
Nominal Concentration (mg/L) | Measured Concentration (mg/L) | Measured Concentration as a Percentage of Nominal (%) | Immobility | |||
(%) | ||||||
T= 0 h | T= 48 h | T= 0 h | T= 48 h | T= 24 h | T= 48 h | |
2000 | 2029.6 | 2074.5 | 101.5 | 103.7 | 100 | 100 |
1000 | 1001.2 | 1027.8 | 100.1 | 102.8 | 100 | 100 |
500 | 502 | 515.8 | 100.4 | 103.2 | 55 | 80 |
250 | 251.2 | 257.5 | 100.5 | 103 | 5 | 20 |
125 | 124.0a | 129.1b | 99.2 | 103.3 | 0 | 45 |
62.5 | 61.8 | 63.7 | 98.9 | 101.9 | 0 | 50 |
31.3 | 30 | 32 | 96 | 102.4 | 0 | 40 |
15.6 | 15.3 | 15.8 | 97.9 | 101.1 | 0 | 20 |
7.8 | 8.4 | 7 | 107.5 | 89.6 | 0 | 0 |
0 | < MDLc | < MDL | N/Ad | N/A | 0 | 0 |
a Average
of duplicate analysis. RPD = 1.2%
b Average of duplicate analysis. RPD = 1.4%
c Method Detection Limit = 0.7 mg/L
d Not Applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the definitive test Nr. 2 the 24-hour and 48-hour EC50s for S-10713 were estimated to be 463 mg/L and 121 mg/L, respectively.
- Executive summary:
The toxicity of S-10713 to aquatic invertebrates was assessed according to OECD guideline No. 202 and GLP principles. Nominal exposure concentrations were the following:
Range finding test - Control, 1, 10, 100 and 1000 mg/L
Definitive tests:
Nr. 1: 0, 10, 20, 40, 80, 160 and 320 mg/L;
Nr. 2: 0, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000 and 2000 mg/L.
Measured water concentrations relative to nominal concentrations varied less than 20% for all tests. Therefore, results are presented in nominal concentrations.
In the definitive test Nr. 2 the 48 h EC50 (immobility) for S-10713 was 121 mg/L.
All criteria for acceptability of the test were met and the present toxicity study is classified as reliable without restrictions according to OECD guideline No. 202.
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