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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well-documented, non-GLP but guideline-conform study report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Chromium trioleate
EC Number:
244-256-3
EC Name:
Chromium trioleate
Cas Number:
21178-63-2
Molecular formula:
C18 H34 O2 .1/3 Cr
IUPAC Name:
chromium(3+) tris((9Z)-octadec-9-enoate)
Details on test material:
Name of the test substance used in the study report: Chrom(III)-Oleat

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
Mean body weight of the animals: male 3.6 kg, females 3.3 kg
Singly housing in stainless steel cages with wire mesh walk floors (floor area: 40 x 51 cm; no bedding in the cages - sawdust in the waste trays). The rabbits were identified via ear tattoo. The animals were housed in fully air-conditioned rooms with a temperature ranging from 20 - 24°C and a relative humidity of 30 - 70%. The day/night rhythm was 12 h dark and 12 h light. The animals were offered a standardized rabbit diet (about 130 g per animal and per day) and drinking water (about 250 ml tap water per animal and day).

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
0.1 ml
Duration of treatment / exposure:
24 h
Observation period (in vivo):
8 resp. 9 days
Number of animals or in vitro replicates:
3
Details on study design:
Acclimatization period: at least 8 days before the beginning of the study under the same housing conditions as during the test period.
Application site: single application to the conjunctival sac of the right eyelid; the test substance was not washed out.
Readings: 1 h, 24 h, 48 h, 72 h and 8 d after application.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
0
Max. score:
0
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
0
Max. score:
0
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
1.3
Max. score:
2
Reversibility:
fully reversible within: 8-9 days
Remarks on result:
other: animal mean scores: 1.0 in 1/3 animals, 1.3 in 1/4 animals, 1.7 in 1/3 animals
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
0.6
Max. score:
2
Reversibility:
fully reversible within: 8-9 days
Remarks on result:
other: animal mean scores: 0 in 1/3 animals, 0.3 in 1/3 animals, 1.3 in 1/3 animals

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information