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EC number: 806-734-5 | CAS number: 109828-01-5
* 6 values
An in-vitro study for predicting non-specific, corrosive potential of Additin E 459 by using reconstructed human skin (RHS) EST-1000 was performed in accordance with OECD TG 431 and with EC guideline (amending Concil Direcrive 67/548/EEC B 40 Skin corrosion). Additin E 459 was tested undiluted (25 mg per insert, plus 50 µl 0.9 % NaCl to moisten and to ensure good contact with the skin). An incubation time of 3 min or of 60 min was used to determine time related toxic effects. After incubation the MTT reduction assay was performed to measure the cell viability which was 47 % and 37 % , respectively. Thus, the results show that a corrosive property of the test item was determined by the assay used.
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