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EC number: 806-734-5 | CAS number: 109828-01-5
A study was performed to assess the adverse effects of Additin E 459 on the growth rate (= rate of increase in cell density with time) and the yield (= biomass at time t minus initial biomass) of the planktonic freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations.
The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006).
Exponentially growing algal cells were exposed for a period of 72 hours to a limit test concentration of nominally 100 mg/L of Additin E 459 dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer.
The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. Growth rates were also used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration according to STUDENT-t test for Homogeneous Variances. The following values were determined:
ErC 50* (0-72 h): > 100
ErC 10* (0-72 h): > 100
NOEC [r]* (ta 0.05): = 100
LOEC [r]* (ta 0.05): > 100
No toxic effects against algae were observed at a limit test concentration of 100 mg/L under exposure conditions.
* Reduction of growth rate (ErCx, NOEC [r]) is the preferred endpoint according to OECD 201 and for regulatory purposes in the EU. Results relating to yield (EyCx, NOEC [y]) were calculated to fulfil regulatory requirements in some countries (but not in the EU) and are given in the results section of this report.
The results are expressed in terms of nominal concentrations. Effective concentrations correspond to 104.8 % of nominal values at 0 hours, and to 99.0 % of nominal values at 72 hours.
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