triphenyl(((1r,4r)-4-((E)-prop-1-en-1-yl)cyclohexyl)methyl)phosphonium iodide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1171067-76-7

Cas Number:

1171067-76-7

IUPAC Name:

1171067-76-7

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

To determine the stability of the test item during the test period the concentrations were measured at test start, at 24 hours and at the end of
exposure.

Test solutions

Vehicle:

yes

Details on test solutions:

In order to get a saturated solution a surplus of the test item was dispersed in test water (100 mg/l) and stirred for 24 hours. The solid particles
were separated by filtration and the clear solution was used for the test. Separate formulations were prepared for each concentration Ievel by dilutingthe saturated solution of the test item with test water. On the basis of a 1 : 10 dilution the further concentrations were arranged in a geometric
series with the separation factor 1.6. The dilution steps covered a concentration range from 0.6 to 7.3 mg/l (table 1). For test group 5 no analytical
result at test start was available. Therefore the value was estimated by means of the separation factor and the data at 24 and 48 hours.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test animal was the water flea species Daphnia magna. Daphnia magna is recommended by the guidelines as a representative test organism for
aquatic invertebrates. They were bred within the laboratory by parthenogenesis and the individuals used were between 6 and 24 hours old.
Breeding conditions:
Light: 15 hours light, 9 hours dark
Feeding: at least 6 days per week with alga and yeast
Test water: aerated reconstituted water
Aeration: none
Temperature: 18.0 - 20.0°C

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

c (Ca++)= 2.04 mmol/L
c (Mg++)= 0.50 mmol/L
CaC03 = 254 ppm

Test temperature:

18.7 - 18.9°C

pH:

8.0 - 8.1

Dissolved oxygen:

8.9 - 9.0 mg O2/L

Nominal and measured concentrations:

measured 0.6, 1.1, 2.0, 2.9, 4.0 and 7.3 mg/L

Details on test conditions:

Duration: 48 hours
Procedure: static test, the test medium remained unchanged throughout the duration of the test.
Light: 15 hours light, 9 hours dark
Feeding: no feeding during the test period
Test water: aerated reconstituted water
c (Ca++)= 2.04 mmol/L
c (Mg++)= 0.50 mmol/L
CaC03 = 254 ppm
Oxygen concentration: at least 8.9 mg/l, the test vessels were not aerated.

Reference substance (positive control):

yes

Remarks:

potassium chromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.6 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.5 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

2.9 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- The mortality in the control did not exceed 10 per cent at the end of the test.
- The dissolved oxygen concentration was at least 8. 9 mg/l.
- The concentration of the test item did not change during the test period.

Results with reference substance (positive control):

EC50 (24 hours) = 1.7 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 h EC50 was determined to be 1.5 mg/L.