Benzamide, 3-amino-N-[4-(aminocarbonyl)phenyl]-4-methoxy-, hydrochloride (1:?)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-amino-N-(4-carbamoylphenyl)-4-methoxybenzamide hydrochloride

EC Number:

618-990-3

Cas Number:

939961-06-5

Molecular formula:

C15H15N3O3xClH

IUPAC Name:

3-amino-N-(4-carbamoylphenyl)-4-methoxybenzamide hydrochloride

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Substance was not completed dissloved. Therefore, for the analytics samples of the suspension were taken and the total content was determined.
Samples were taken at 0 hours as well after 48 and 96 hours.

Test solutions

Vehicle:

no

Details on test solutions:

Homogenization with ultrasonic and utra-turrax,
not completely dissolved

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: 2.6 - 3.4 cm:
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke, Melle; twice a day, ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

test groups: 21.3 - 21.8 °C
controls: 21.0 - 22.0 °C

pH:

test groups: 6.8 - 7.7
controls: 7.9 - 8.3

Dissolved oxygen:

test groups: 7.1 - 9.1 mg/L
controls: 7.6 - 9.7 mg/L

Nominal and measured concentrations:

nominal measured
(mg/L) (mg/L)
220 251.7
500 505

Details on test conditions:

TEST SYSTEM
- Test vessel: open glass vessel, 30 cm x 22 cm x 24 cm, calibrated for 10 liters
- Aeration: during test : no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours dark / 12 hours light
- Light intensity: 700 Lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Observations:
projecting opercula, panting, lateral and supine position, swim posture tail-heavy,

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance has not to be classified for toxicity to fish since the LC50 is greater than 500 mg/L.

Executive summary:

The substance was tested for acute toxicity to the fish Brahydanio rerio for 96 hours according to OECD guideline 203. The test was performed in two groups with 0 mg/L (control) and 220 mg/L and with 0 mg/L and 500 mg/L. After 24 hours 10% lethality occurred in the 500 mg/L group. For each test 10 fish were used. Observations during the study were projecting opercula, panting, lateral and supine position, tail heavy swim posture.

Based on the results the LC50 can be determined as greater than 500 mg/L