Registration Dossier
Registration Dossier
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EC number: 634-657-5 | CAS number: 65962-45-0
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Acutely harmful to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 31.6 mg/L
Additional information
No experimental data are available for 4,4'-diamino-3,3',5,5'-tetramethyldicyclohexylmethan (CAS 65962 -45 -0). Therefore a read-across approach to two structurally similar substances was performed.
The key study was performed on 2,2'-dimethyl-4,4'-methylenebis(cyclohexylamin) (CAS 6864-37-5) according to OECD 203 (NITE 2002) with Oryzias latipes. The 96-LC50 was determined to be 22.4 mg/L, the NOEC was 16 mg/L and the LC100 was 30 mg/L. The result is supported by a static-test study on CAS 6864-37-5 according to German industrial standard (DIN) 38412/15 (BASF 1988) performed with Leuciscus idus as test species. A 96-h LC50 of 31.6 mg/l was determined for non-neutralized samples (expressed as geometrical mean). The NOEC was determined to be 21.5 mg/l and the LC100 46.4 mg/l. Because DMD changes the pH in water, a neutralized sample with the highest test concentration (100 mg/l) was tested in addition. For this neutralized sample a diminution of the toxic effects was observed.
An additional supporting study is a test performed on 4,4'-methylenebis-cyclohexanamine (CAS 1761-71-3) also performed according to German Industrial Standard DIN 38412, Part 15 using Leuciscus idus as test species (BASF AG, 1988; report no. 10F0562/875273). The 96 -h LC50 of 68.1 mg/L was determined for non-neutralised samples. A neutralised sample lead to a significant reduction of the mortality (96 -h LC50 > 100 mg/L).
In conclusion, the toxic potential for 4,4'-diamino-3,3',5,5'-tetramethyldicyclohexylmethan (CAS 65962 -45 -0) is assessed as acutely harmful to fish.
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