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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented study report which meets basic scientific principles, only a 10% aqueous substance preparation was tested.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1975
Report Date:
1975
Reference Type:
other: OECD SIDS
Title:
1-Chloro-2-nitrobenzene, CAS: 88-73-3
Author:
OECD SIDS
Year:
2001
Bibliographic source:
SIDS Initial Assessment Report for SIAM 13

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Patch-test according to FDA guidelines
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Physical state: yellow crystals

Test animals

Species:
rabbit
Strain:
Himalayan
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 1.5-2.0 kg
- Housing: individually
- Diet: Mischfutter ERKA 8300 der Futtermittelwerke Robert Koch oHG/ Hamm, ad libitum
- Water: tap water, ad libitum

Test system

Type of coverage:
semiocclusive
Preparation of test site:
other: intact and abraded skin
Vehicle:
other: sesame oil
Controls:
no
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL
- Concentration (if solution): 10% in sesame oil
Duration of treatment / exposure:
24 h
Observation period:
up to 72 h
Number of animals:
6
Details on study design:
TEST SITE
- Area of exposure: 2.5 x 2.5 cm
- Type of wrap if used: surgical gauze, kept in place using adhesive tape, was covered with a 6-8 cm wide PVC film after which the bodies of the animals were wrapped in elastic binding

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal: 1 - 4
Time point:
other: 24 h
Score:
1
Max. score:
4
Reversibility:
fully reversible within: 48 h
Remarks on result:
other: intact and abraded skin
Irritation parameter:
erythema score
Basis:
animal: 5, 6
Time point:
other: 24 h
Score:
0
Max. score:
4
Remarks on result:
other: intact and abraded skin
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 48, 72 h
Score:
0
Max. score:
4
Remarks on result:
other: intact and abraded skin
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
0
Max. score:
4
Remarks on result:
other: intact and abraded skin
Irritant / corrosive response data:
Weak erythema (score 1) was observed in 4 out of 6 animals at both intact and abraded skin sites. It was fully resolved at 48 hours observation.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information