Cyclododeca-1,5,9-triene

Specific investigations: other studies

Administrative data

Endpoint:

biochemical or cellular interactions

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity.

Author:

Sparnins VL, Lam LKT and Wattenberg LW

Year:

1984

Bibliographic source:

Biochem. Biophys. Res. Commun. 120, 637-640.

Materials and methods

Principles of method if other than guideline:

see reference

Type of method:

in vivo

Endpoint addressed:

basic toxicokinetics

Test material

Test material information

Constituent 1

Reference substance name:

(1E,5E,9Z)-cyclododeca-1,5,9-triene

EC Number:

211-893-3

EC Name:

(1E,5E,9Z)-cyclododeca-1,5,9-triene

Cas Number:

706-31-0

IUPAC Name:

cyclododeca-1,5,9-triene

Constituent 2

Reference substance name:

trans-trans-cis-1,5,9-cyclododecatriene

IUPAC Name:

trans-trans-cis-1,5,9-cyclododecatriene

Details on test material:

trans-trans-cis-1,5,9-cyclododecatriene (CAS RN 706-31-0) from Aldrich Chemical Company, purity not reported

Test animals

Species:

mouse

Strain:

other: ICR/Ha mice

Sex:

female

Details on test animals or test system and environmental conditions:

Age: 7 weeks
Number: 5-10 animals per group

Administration / exposure

Route of administration:

oral: feed

Details on exposure:

Control: diet
TREATMENT
- 30 or 50 µmol/g in diet for 2 weeks

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

2 weeks

Frequency of treatment:

test item in feed

Doses / concentrations

Remarks:

Doses / Concentrations:
30 or 50 µmol/g in diet
Basis:
nominal in diet

No. of animals per sex per dose:

Number: 5-10 animals per group

Control animals:

yes

Examinations

Examinations:

EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate

Results and discussion

Details on results:

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated. With trans-trans-cis-1,5,9-cyclododecatriene as test substance, the GST  activity in the livers was increased 3.79-, 3.78- and 3.82-fold,  respectively, in three experiments. The increases in the intetinal GST  activity were of similar magnitude.

Any other information on results incl. tables

-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                1.96 +- 0.16           0.61 +- 0.04
Cyclododecatriene   7.42 +- 0.53*          2.77 +- 0.29*

None                2.40 +- 0.10           0.65 +- 0.01
Cyclododecatriene   9.06 +- 0.04*          1.74 +- 0.15*

None                1.39 +- 0.05           0.27 +- 0.01
Cyclododecatriene   5.31 +- 0.23*          1.10 +- 0.09*
     * = p <0.005