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EC number: 700-891-2 | CAS number: -
In a static acute toxicity test performed according to OECD guideline 201, the alga Pseudokirchneriella subcapitata was exposed for 72 hours to three nominal concentrations ranging between 1 and 100 mg/L of 2237 JG/L. The initial cell concentration was approximately 10 000 cells/mL.
Since the test item was insoluble in the tested medium, the use of Water Accommodated Fractions (WAF) for the preparation of the test media was necessary.
The test item stability was checked by the determination of Alkyl Poly Glucosid (APG) in fresh and old WAF (GC-FID; non GLP data). Because of the instability of APG under light conditions, actual concentrations of APG were not in good agreement with the initial concentrations (-42% and -61% after 72 hours by comparison to initial measurements for 10 and 100 mg/L test concentrations respectively).
The results obtained showed that at the highest tested concentration of 100 mg/L (nominal concentration), a toxicity of the test item with an inhibition of the average specific growth rate in comparison to the control equal to 22.1% during the first test and 17.3% during the second test.
ErC50(72 h) > 100 mg/L (nominal)
NOEC (72 h) > 10 mg/L (nominal)
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