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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(2-{1-[(2-methoxyphenyl)carbamoyl]-2-oxopropyl}diazen-1-yl)benzoic acid
EC Number:
683-287-0
Cas Number:
877670-90-1
Molecular formula:
C18H17N3O5
IUPAC Name:
2-(2-{1-[(2-methoxyphenyl)carbamoyl]-2-oxopropyl}diazen-1-yl)benzoic acid

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19.8-20.1°C
pH:
7.12-7.64
Dissolved oxygen:
6.8-6-9 mg/L
Nominal and measured concentrations:
1.78 mg/L (measured)
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
> 1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
1.78 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of this Daphnia magna acute immobilisation study the
calculated and observed endpoints for the effect of ANTHRANILIC ACIDAAOA
were the followings:
24 h NOEC value: 1.78 mg test item/L
24 h LOEC value: >1.78 mg test item/L
The 24h EC50 value: >1.78 mg test item/L
48 h NOEC value: 1.78 mg test item/L
48 h LOEC value: >1.78 mg test item/L
The 48h EC50 value: >1.78 mg test item/L
The 48h EC100 value: >1.78 mg test item/L
The 48h EC0 value: 1.78 mg test item/L
Executive summary:

The purpose of this study was to evaluate the influence of the test item

ANTHRANILIC ACID-AAOA on the mobility of Daphnia magna as a surrogate

endpoint for survival. Young Daphnia were exposed in a static test to aqueous test

media containing the test item.

Because no significant toxic response was observed in the preliminary range-finding

test at the highest applied concentration level (at the limit of solubility in the test

medium, S.S./1), only this concentration and a control was tested in a limit-test.

(Limit-test according to the Commission Directive 92/69/EEC).

The corresponding measured mean test item concentration was: 1.78 mg/L.

Four vessels were used in the test concentration and in the control group. Each group

comprised twenty Daphnia, five in each of the four replicate vessels, each containing

40 ml test dilution.

Under the conditions of this Daphnia magna acute immobilisation study the

calculated and observed endpoints for the effect of ANTHRANILIC ACIDAAOA

were the followings:

24 h NOEC value: 1.78 mg test item/L

24 h LOEC value: >1.78 mg test item/L

The 24h EC50 value: >1.78 mg test item/L

48 h NOEC value: 1.78 mg test item/L

48 h LOEC value: >1.78 mg test item/L

The 48h EC50 value: >1.78 mg test item/L

The 48h EC100 value: >1.78 mg test item/L

The 48h EC0 value: 1.78 mg test item/L