1,1'-[methylenebis(oxy)]dibutane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

other information

Study period:

29. Aug 1968 - 09. Sep 1968

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Principles of method if other than guideline:

BASF test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-[methylenebis(oxy)]dibutane

EC Number:

219-909-0

EC Name:

1,1'-[methylenebis(oxy)]dibutane

Cas Number:

2568-90-3

Molecular formula:

C9H20O2

IUPAC Name:

1,1'-[methylenebis(oxy)]dibutane

Details on test material:

- Name of test material (as cited in study report): Butylal; Formaldehyddibutylacetal
- Physical state: liquid
- Analytical purity: 98-99 %

Test animals

Species:

rat

Strain:

other: US-rats

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: male: 188 g (mean); female: 158 g (mean)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 30% aqueous emulsion in Traganth

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2, 20 and 30 %

Doses:

200, 1600, 3200, 6400 ml/kg bw (168, 1344, 2688, 5376 mg/kg bw; conversion into mg/kg is based on the density d= 0.84 g/cm3 according to GESTIS database)

No. of animals per sex per dose:

10

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 376 mg/kg bw

Remarks on result:

other: mg/kg bw; conversion into mg/kg is based on the density d= 0.84 g/cm3 according to GESTIS database

Mortality:

5376 mg/kg bw: one male died within 24 h.

Clinical signs:

5376 mg/kg bw:
5 min after application irregular respiration, masticatory movement, watery mouth discharge, high stepping gait, piloerection.
24 h post application irregular respiration, latero-abdominal position and apathy were observed.

2688 mg/kg bw: on the day of application same symptoms as higher dose group and 24 h post application irregular respiration were noted.

168 and 1344 mg/kg bw: intermittent respiration, squatting posture, masticatory movements.

Body weight:

no data

Gross pathology:

Died animals:
1x increased nose secretion;
Lung: distended and blood filled;
Intragastric tract: atony with aqueous-mucous content;
Bladder: dilatation

Sacrificed animals:
5x bronchitis and bronchiectasis; 2x bronchial pneumonia; 4x intravesicular clot and moderate dilatation of the bladder.

Any other information on results incl. tables

Mortality:

Dose (mg/kg bw)	Gender	1 h	24 h	48 h	7 days
5376	male	0/10	1/10	1/10	1/10
5376	female	0/10	0/10	0/10	0/10
2608	male	0/10	0/10	0/10	0/10
2608	female	0/10	0/10	0/10	0/10
1344	male	0/10	0/10	0/10	0/10
1344	female	0/10	0/10	0/10	0/10
168	male	0/10	0/10	0/10	0/10
168	female	0/10	0/10	0/10	0/10

The test substance caused signs of slight systemic toxicity after a single ingestion but mortality only in high doses.