4-amino-6-chlorotoluene-3-sulphonic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards without analytical test item concentration verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-amino-6-chlorotoluene-3-sulphonate

EC Number:

229-596-2

EC Name:

Sodium 4-amino-6-chlorotoluene-3-sulphonate

Cas Number:

6627-59-4

IUPAC Name:

sodium 2-amino-4-chloro-5-methylbenzenesulfonate

Details on test material:

- Name of test material (as cited in study report): Chlor-p-Toluidinsaeure (sodium salt)
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The doses were chosen based on a range finding study.
- The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West aquarium GmbH & Co. KG, 3422 Bad Lauterberg, Germany
- Length at study initiation: 2.8 cm (range: 2.4 - 3.1)
- Weight at study initiation: 0.4 g (range: 0.2 - 0.6)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day


ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 23 ± 1°C
Acclimation to test water and test temperature: 7 days

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 8.3
- Water temperature: 20 - 25 °C
- Duration of housing: about 2 months
- Medical treatment: None
- Feeding: TETRA Min Standard feed for aquarium fish, TETRA - Werke, 4520 Melle, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 2.5 mM
Acid capacity: 0.8 mM

Test temperature:

20 ±1 °C

pH:

7.9 - 8.2

Dissolved oxygen:

7.6 - 8.7 mg/L

Nominal and measured concentrations:

Nominal concentrations: 100 and 1000 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L for the controls, 5 L for the test concentrations
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.8 g fish /L (controls 0.4 g/L)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 0, 2, 24, 48, 72, and 96 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no symptoms detectable

Applicant's summary and conclusion

Validity criteria fulfilled:

yes