Eldew APS-307

Partition coefficient

Administrative data

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Between 23 January 2009 and 27 February 2009.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Date of GLP inspection: 19/08/2008 Date of Signature on GLP certificate: 04/03/2009

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Eldew APS-307
- Substance type: Organic (UVCB)
- Physical state: Pale yellow waxy solid
- Analytical purity: 100%
- Lot/batch No.: 809114
- Stability under test conditions: stable
- Date received: 22 December 2008
- Storage condition of test material: Room temperature, in the dark

Study design

Analytical method:

high-performance liquid chromatography

Results and discussion

Partition coefficient

Type:

log Pow

Partition coefficient:

> 6.5

Temp.:

30 °C

pH:

6.4

Details on results:

Results:
Preliminary estimate:
Approximate solubility in n-octanol: >48.5% w/w
Aproximate solubility in water: <1.4 x 10E-4 % w/w
Approximate Pow: >3.6 x 10E5
Log10 Pow: >5.54

Results from Definitive Test:
Mean Log Pow: >6.50
Partition coefficient: >3.162 x 10E6.

Any other information on results incl. tables

Definitive test:

Calibration

The retention times (RT) in minutes of the deadtime and the retention times, capacity factors (k) and log₁₀Pow values for the reference standards are shown in the following tables below:

Dead time	RT (mins) (Injection 1)	RT (mins) (Injection 2)	Mean RT (mins)
Thiourea	1.557	1.556	1.557

Stanadard	RT injection1	RT injection 2	Mean RT (mins)	Capacity factor (k)	Log10K	Log10Pow
2 -Butanone	1.830	1.832	1.831	0.176	-0.754	0.3
Benzonitrile	2.039	2.042	2.041	0.311	-0.507	1.6
Toluene	3.435	3.440	3.438	1.208	8.22 x 10^-2	2.7
Naphthalene	3.878	3.884	3.881	1.493	0.174	3.6
Phenanthrene	5.816	5.820	5.818	2.738	0.437	4.5
DDT	18.337	18.322	18.330	10.776	1.032	6.5

Partition coefficient of sample: The retention times, capacity factors and log10 Pow values determined for the sample are shown in the table below:

Peak No.	Injection	RT (mins)	Capacity factor (k)	Log10K	Log10Pow	Overall Log10Pow
1	1	37.044	22.800	1.358	>6.50	>6.50
1	2	37.050	22.803	1.358	>6.50	>6.50
2	1	37.413	23.037	1.362	>6.50	>6.50
2	2	37.419	23.040	1.362	>6.50	>6.50
3	1	38.167	23.521	1.371	>6.50	>6.50
3	2	38.175	23.526	1.372	>6.50	>6.50

Discussion:

Substances having a log_l0 P_ow greater than 3 are regarded as having the potential to bioaccumulate in the environment. Identification of the major peaks of interest (decyltetradecyl myristoyl beta alaninate and phytosteryl myristoyl beta alaninate) was performed using analytical details provided by the Sponsor. In the absence of any dissociating functional groups within the environmentally relevant pH range, no specific manipulation of the mobile phase pH was necessary to ensure that the test material was analysed in a non-ionised form. Insignificant differences in the test material peak retention times were observed following the subtraction of a solvent blank (methanol) injection. However this had no impact on the limit value obtained for the partition coefficient result.

To conclude, the partition coefficient of the test material has been determined to be greater than 3.16 x 10⁶, log₁₀ P_ow >6.50.

Applicant's summary and conclusion

Conclusions:

The partition coefficient of the test material has been determined to be > 3.16 x 10E6, log Pow >6.50.

Executive summary:

Method

The determination was carried out using the HPLC Method, Method A8 Partition Coefficient of Council Regulation (EC) No 440/2008 of 30 May 2008.

 

Mean Log₁₀ P_ow >6.50

Partition Coefficient: > 3.162 x 10⁶

Conclusion

To conclude, the partition coefficient of the test material has been determined to be greater than 3.16 x 10⁶, log₁₀ P_ow >6.50