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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well performed study but only weak documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,1'-ethane-1,2-diylbis(4-nitrobenzene)
EC Number:
212-001-5
Cas Number:
736-30-1
Molecular formula:
C14H12N2O4
IUPAC Name:
1,1'-ethane-1,2-diylbis(4-nitrobenzene)

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
A mixed population of activated sewage sludge microorganisms.
Source: The aeration Stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.
Preparation:
The sample was maintained at 21°C with continuous aeration
and used on the day of collection. The pH was buffered to
pH 7.4 with sodium hydroxide solution and the suspended
solids level adjusted to 3.8 gIl prior to use.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: O2 consumption rate
Details on results:
Concentration 02 consumption rates %Inhibition
(mg/l) mg/02/l/mi n
3 hour contact time
Control R1 0.37
Control R2 0.35
Test 100 R1 0.38 <6>
Test 100 R2 0.36 0
Test 100 R3 0.36 0
Reference 3.2 0.34 6
Reference 32 0.09 75
Results with reference substance (positive control):
see above

Applicant's summary and conclusion

Conclusions:
The EC0 value is 100 mg/L and EC50 is > 100 mg/L.