Octahydrocyclopenta[c]pyrrole

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

This study was performed following GLP requirements, but as a screening test and without analytical monitoring. Therefore, the test do not totally comply with the specific testing guideline but is well documented and scientifically acceptable. For this reason, we considere it as Klimish 2.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Octahydrocyclopenta[c]pyrrole

EC Number:

227-114-5

EC Name:

Octahydrocyclopenta[c]pyrrole

Cas Number:

5661-03-0

Molecular formula:

C7H13N

IUPAC Name:

octahydrocyclopenta[c]pyrrole

Test material form:

other: liquid

Details on test material:

Colourless liquid, stable, soluble in water, stored at room temperature.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

A stock solution of appropriate concentrations in dilution water was prepared.
Dilution water : deionised water with an enrichment of certain minerals (as required in the guidelines).
After preparation, the dilution water is aerated and the pH is adjusted to 7.8 +/-0.2.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Female daphnia magna
Variety : STRAUS
Strain : Berlin.
Age : between 0 and 24 hours.

Study design

Test type:

static

Water media type:

freshwater

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

20 +/- 2°C.

pH:

7.8 +/-0.2.

Dissolved oxygen:

O2 concentration at the beginning of the test = 9.3 mg/L.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence interval not determinable

Any other information on results incl. tables

The main study was performed using three concentration containing 1, 10, 100 mg/L (nominal).

Twenty daphnia were exposed to the test item for 48 hours in a static system.

After 24 and 48 hours, the immobilized daphnia were counted.

The concentrations showed no toxicity. No animals were immobilized in the control.

In the current positive control test : the value of 24h-EC50i of potassium dichromate was determined as 1.3 mg/L, lying within the required range of 06 -1.7 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results were determined for the test item :
24h-NOEC = 100 mg/L
48h-NOEC = 100 mg/L
24h-EC50i > 100 mg/L
48h-EC50i > 100 mg/L