Octahydrocyclopenta[c]pyrrole

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: All tests were made in accordance with the 'revised OECD Principes of Good Laboratory Practices', Guidelines OECD, Directive 2004/10/EC and Chemikaliengesetz of federal republic of Germany.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Octahydrocyclopenta[c]pyrrole

EC Number:

227-114-5

EC Name:

Octahydrocyclopenta[c]pyrrole

Cas Number:

5661-03-0

Molecular formula:

C7H13N

IUPAC Name:

octahydrocyclopenta[c]pyrrole

Test material form:

other: liquid

Details on test material:

Colourless liquid, stable, soluble in water, stored at room temperature.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge from biologic sewage treatment plant was used. The chosen plant is treating mostly domestic sewage.
The sludge was taken from the activation basin of the ESn (Stadtensorgnung Neustadt) sewage treatment plant, im Altenschemel. Batch n°20100305
date of collection : 05 march 2010.
The sludge was filtrated, washed with tap water, twice, then washed with and resuspended in the test medium. It was then aerated for >12hours. The dry matter was determined with 3880mg suspended solids/liter.
All flasks containing medium and inoculum were aerated for 72 hours, CO2-free, moistened air to purge the system of CO2.

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Results and discussion

Any other information on results incl. tables

All validity parameters and values are presented in the following table :

parameter	criterion	found	assessment
IC content of test item solution in medium	=<5% of TC	<1%	valid
CO2 emitted by the controls	<70mg/L	9.8 mg/L	valid
difference within replicates	=<20%	4.3%	valid
degradation of positive control > 60%	<14 days	9 days	valid
degradation in the toxicity flask on day 14	>25%	33%	valid

Degradation behaviour of positive control an toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence. No sign of degradation were visible.

All validity criteria were met. Degradation behaviour of positive control an toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence. No sign of degradation were visible.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: NOT READILY BIODEGRADABLE

Conclusions:

The degree of biodegradation reached 7.3% after 28 days.
No 10-days window could be identified and the pass level of 60% given in the OECD guideline was missed.
No abiotic degradation was observed.