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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
2000 - 2002.
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A standard NTP (National Toxicology Program) protocol. Equivalent to OECD 471.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: Standard NTP Protocol.
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
yes
Remarks:
(only three Salmonella strains were tested)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-vinylpyridine
EC Number:
202-879-8
EC Name:
2-vinylpyridine
Cas Number:
100-69-6
Molecular formula:
C7H7N
IUPAC Name:
2-ethenylpyridine
Details on test material:
- Name of test material (as cited in study report): 2-vinylpyridine

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: S. typhimurium TA 100, TA 1535 and TA 98.
Metabolic activation:
with and without
Metabolic activation system:
Induced male Sprague Dawley rat liver S9 mix and induced male Syrian hamster liver S9 mix.
Test concentrations with justification for top dose:
TA 1535: 0, 100, 333, 1000, 2000, 3333, 4000 μg/plate.
TA 98 and TA 100: 0, 100, 333, 1000, 2000, 3333, 5000, 10000 μg/plate.
Vehicle / solvent:
- Vehicle/solvent used: Dimethyl Sulfoxide.
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
Remarks:
Dimethyl Sulfoxide.
Positive controls:
yes
Positive control substance:
sodium azide
other: 2-aminoanthracene, 4-nitro-o-phenylenediamine.
Remarks:
(sodium azide: without metabolic activation; 2-aminoanthracene, 4-nitro-o-phenylenediamine: with metabolic activation).
Details on test system and experimental conditions:
METHOD OF APPLICATION: preincubation method.

DURATION
- Preincubation period: 20 minutes at 37 ºC.

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium, other: S. typhimurium TA 100, TA 1535 and TA 98.
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(slightly toxicity at ≥ 10000 µg/plate)
Vehicle controls validity:
valid
Untreated negative controls validity:
not applicable
Positive controls validity:
valid
Species / strain:
S. typhimurium, other: S. typhimurium TA 100 and TA 1535.
Metabolic activation:
with
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(slightly toxicity at ≥ 5000 µg/plate)
Vehicle controls validity:
valid
Untreated negative controls validity:
not applicable
Positive controls validity:
valid
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with
Genotoxicity:
ambiguous
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(slightly toxicity at ≥ 5000 µg/plate)
Vehicle controls validity:
valid
Untreated negative controls validity:
not applicable
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive with metabolic activation (induced male Syrian hamster liver S9).

The test substance is considered to be genotoxic in vitro.
Executive summary:

A standard NTP (National Toxicology Program) protocol was performed to investigate the mutagenicity of 2-vinylpyridine (test method equivalent to OECD 471). Different concentrations of the test substance were tested in Salmonella typhimurium strains TA 100, TA 1535 and TA 98 by the preincubation assay (37 ºC, 20 min) with and without metabolic activation (induced male Syrian hamster liver S9 mix and induced male SD rat liver S9 mix). Positive and negative controls confirmed the sensitivity of the test system. In the presence of exogenous metabolic activation, Salmonella strains TA 100 and TA 1535 gave positive results, while S. typhimurium TA 98 gave ambiguous results. Slightly toxicity was found at concentrations of 5000 µg/plate and above. From the results of the present study, the test substance is considered to be genotoxic in vitro.

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