Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate
EC Number:
275-177-2
EC Name:
Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate
Cas Number:
71077-34-4
Molecular formula:
C14H14N2O4S.Na
IUPAC Name:
sodium 4-[(3-amino-4-methylbenzoyl)amino]benzenesulfonate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida Migula, DSM 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
22°C
Reference substance (positive control):
yes
Remarks:
2,4-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
other: inhibitionrate
Effect conc.:
1 328 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: -24.9%
Duration:
16 h
Dose descriptor:
other: inhibitionrate
Effect conc.:
664 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: -11.1%
Duration:
16 h
Dose descriptor:
other: inhibition rate
Effect conc.:
332 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: -9.9%
Details on results:
The substance supports an slight increase of the growth of the bacteria
Results with reference substance (positive control):
2,4-dichlorophenol
30 mg/L : 11.1 % growth inhibition
90 mg/L: 79.7% growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for bacteria is greater then 1328 mg/L
Executive summary:

A growth inhibition test according DIN 38 412 Teil 8 for Pseudomonas putida was performed with the substance using concentrations of 1328, 664 and 332 mg/L.

There was inhibition observed but a slight increase of the growth of the bacteria