1H-Perylo[3,4-cd]pyridine-1,3(2H)-dione

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, no deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1H-benzo[10,5]anthra[2,1,9-def]isoquinoline-1,3(2H)-dione

EC Number:

608-929-9

Cas Number:

33955-44-1

Molecular formula:

C22H11NO2

IUPAC Name:

1H-benzo[10,5]anthra[2,1,9-def]isoquinoline-1,3(2H)-dione

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): PERYLMONOIMID PULVER
- Molecular formula (if other than submission substance): C22 H11 N 02
- Molecular weight (if other than submission substance): 321.33
- Physical state: solid
- Analytical purity: 79.0 g/100 g by ŠH-NMR spectroscopy using the internal standard method
- Purity test date: 06.07.2000
- Lot/batch No.: Partie 6/99
- Stability under test conditions: given
- Special storage condition of test material: none
- Water solubility: insoluble

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Handling of a poorly water-soluble test substance:
The test substance was added in the required amount to the test vessel with about 180 ml deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. Then synthetic medium was added , the pH-value was adjusted to 7,5 ± 0,5, the inoculum was added and the test started.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

lnoculum:
Activated sludge from Iaboratory wastewater plant treating municipal sewage. Concentration of dry substance 1 g/I.
Preparation of the inoculum:
The inoculum was washed, brought to a concentration of 5.g/1 dry substance and aerated dur ing the
night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/1 dry substance in the
test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

180 min

Test conditions

Test temperature:

20 +/- 2°C

Dissolved oxygen:

>= 2.5 mg/L

Nominal and measured concentrations:

Nominal: 0, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1 g/L dry substance

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20: 1.6 mg/L
EC50: 5.8 mg/L
EC80: 38 mg/L

Reported statistics and error estimates:

not applicable