Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study, no deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): PERYLMONOIMID PULVER
- Molecular formula (if other than submission substance): C22 H11 N 02
- Molecular weight (if other than submission substance): 321.33
- Physical state: solid
- Analytical purity: 79.0 g/100 g by ŠH-NMR spectroscopy using the internal standard method
- Purity test date: 06.07.2000
- Lot/batch No.: Partie 6/99
- Stability under test conditions: given
- Special storage condition of test material: none
- Water solubility: insoluble

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Handling of a poorly water-soluble test substance:
The test substance was added in the required amount to the test vessel with about 180 ml deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. Then synthetic medium was added , the pH-value was adjusted to 7,5 ± 0,5, the inoculum was added and the test started.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
lnoculum:
Activated sludge from Iaboratory wastewater plant treating municipal sewage. Concentration of dry substance 1 g/I.
Preparation of the inoculum:
The inoculum was washed, brought to a concentration of 5.g/1 dry substance and aerated dur ing the
night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/1 dry substance in the
test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
180 min

Test conditions

Test temperature:
20 +/- 2°C
Dissolved oxygen:
>= 2.5 mg/L
Nominal and measured concentrations:
Nominal: 0, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1 g/L dry substance

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC20: 1.6 mg/L
EC50: 5.8 mg/L
EC80: 38 mg/L
Reported statistics and error estimates:
not applicable

Applicant's summary and conclusion