1H-Perylo[3,4-cd]pyridine-1,3(2H)-dione

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study - no analytical monitoring; data were taken from a structural similar substance (CAS 81-33-4)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Perylene-3,4:9,10-tetracarboxydiimide

EC Number:

201-344-6

EC Name:

Perylene-3,4:9,10-tetracarboxydiimide

Cas Number:

81-33-4

IUPAC Name:

isoquino[4',5',6':6,5,10]anthra[2,1,9-def]isoquinoline-1,3,8,10(2H,9H)-tetrone

Details on test material:

- Analytical purity: >95 %
- Water solubility (mg/L): 670

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was mixed with the test medium and homogenised using an ultra turrax.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): floating particles of test substance were visible.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): ca. 4 month
- Length at study initiation (length definition, mean, range and SD): 2.7 - 3.5 cm


ACCLIMATION
- Type and amount of food: Tetra Min, Tetra Werke, Melle, Germany
- Feeding frequency: twice a day

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

21.1 - 22.9°C

pH:

6.9 - 8.1

Dissolved oxygen:

7.0 - 9.3 mg/L

Nominal and measured concentrations:

Nominal test substance concentration:
0, 5000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium (30 x 22 x 24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: =< 1 g Fisch/ l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water ISO/DIS 7346/1


OTHER TEST CONDITIONS
- Photoperiod: 12:12 h day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 0, 2-4, 24, 48, 72 and 96 hours

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)