Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study - no analytical monitoring; data were taken from a structural similar substance (CAS 81-33-4)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Analytical purity: >95 %
- Water solubility (mg/L): 670

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was mixed with the test medium and homogenised using an ultra turrax.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): floating particles of test substance were visible.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): ca. 4 month
- Length at study initiation (length definition, mean, range and SD): 2.7 - 3.5 cm


ACCLIMATION
- Type and amount of food: Tetra Min, Tetra Werke, Melle, Germany
- Feeding frequency: twice a day

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
21.1 - 22.9°C
pH:
6.9 - 8.1
Dissolved oxygen:
7.0 - 9.3 mg/L
Nominal and measured concentrations:
Nominal test substance concentration:
0, 5000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquarium (30 x 22 x 24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: =< 1 g Fisch/ l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water ISO/DIS 7346/1


OTHER TEST CONDITIONS
- Photoperiod: 12:12 h day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 0, 2-4, 24, 48, 72 and 96 hours

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion